Ultra-LEAF™ Purified anti-human CD273 (B7-DC, PD-L2) Antibody

Pricing & Availability
Clone
MIH18 (See other available formats)
Workshop
HCDM listed
Other Names
B7-DC, PD-L2, PDL2, B7DC
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MIH18_PURE_CD273_Antibody_102618
Human monocyte-derived dendritic cells were stained with CD273 (clone MIH18) purified (filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by biotin anti-mouse IgG and Streptavidin-PE.
  • MIH18_PURE_CD273_Antibody_102618
    Human monocyte-derived dendritic cells were stained with CD273 (clone MIH18) purified (filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by biotin anti-mouse IgG and Streptavidin-PE.
Cat # Size Price Quantity Avail. Save
345521 100 µg £195
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345522 1 mg £433
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Description

CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25 kD type I transmembrane protein and a member of B7 family within the immunoglobulin receptor superfamily and is expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. Clone MIH18 is reported to block PDL2.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human B7-DC transfected cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IHC, IF, Block - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking4,5, and immunohistochemistry in frozen sections2 and paraffin-embedded formalin-fixed sections6. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 345504).

Application References

(PubMed link indicates BioLegend citation)
  1. Youngnak-Piboonratankit P, et al. 2004. Immunol. Lett. 94:215. (IHC, IF)
  2. Ohigashi Y, et al. 2005. Clin. Cancer. Res. 8:2947. (IHC, IF)
  3. Hobo W, et al. 2010. Blood 25:4501. (FC)
  4. Nagamatsu T, et al. 2009. Hum. Reprod. 24:3160. (Block)
  5. Alvarez IB, et al. 2010. J. Infect. Dis. 202:524. (Block)
  6. Taube JM, et al. 2014. Clin. Cancer Res. 19:5064. (IHC) PubMed
RRID
AB_2783235 (BioLegend Cat. No. 345521)
AB_2783236 (BioLegend Cat. No. 345522)

Antigen Details

Structure
B7 Immunoglobulin superfamily, 25 kD
Distribution

Dendritic cells, liver, few transformed cell lines, subset of macrophages

Function
Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
Ligand Receptor
PD-1
Cell Type
Dendritic cells
Biology Area
Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.
2. Ohigashi Y, et al. 2005. Clin. Cancer. Res. 8:2947.

Gene ID
80380 View all products for this Gene ID
UniProt
View information about CD273 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house unless otherwise indicated. However upon request these can be tested from outside independent laboratories on custom basis.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 10/26/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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