Ultra-LEAF™ Purified anti-human CD140b (PDGFRβ) Antibody

Pricing & Availability
Clone
A17169H (See other available formats)
Other Names
Platelet Derived Growth Factor Receptor Beta, Beta Platelet-Derived Growth Factor Receptor, PDGFR-B, PDGFR β, CD140B, PDGFR1, PDGF-R-B
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17169H_ULEAF_CD140b_Antibody_081919.png
Recombinant human PDGFRβ (Black Circles, Cat. No. 767206) binds to immobilized recombinant human PDGF-BB (Cat. No. 577306) Anti-human PDGFRβ antibody (Purple Squares, Clone A17169H) inhibits the binding in a dose-dependent manner whereas the isotype control (Green Triangles, Cat. No. 400366) does not have this effect. This antibody blocks the binding of 0.3 µg/mL recombinant human PDGFRβ protein to 1 µg/mL of immobilized recombinant human PDGF-BB with an ND50 = 0.08 - 0.6 µg/mL.
  • A17169H_ULEAF_CD140b_Antibody_081919.png
    Recombinant human PDGFRβ (Black Circles, Cat. No. 767206) binds to immobilized recombinant human PDGF-BB (Cat. No. 577306) Anti-human PDGFRβ antibody (Purple Squares, Clone A17169H) inhibits the binding in a dose-dependent manner whereas the isotype control (Green Triangles, Cat. No. 400366) does not have this effect. This antibody blocks the binding of 0.3 µg/mL recombinant human PDGFRβ protein to 1 µg/mL of immobilized recombinant human PDGF-BB with an ND50 = 0.08 - 0.6 µg/mL.
Cat # Size Price Quantity Avail. Save
622803 100 µg £165
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622804 1 mg £437
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Description

The PDGF family includes five members, PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC, and PDGF-DD. Two PDGF receptors (α and β) have been described; they belong to the receptor-tyrosine kinase family, Type III group, which also includes c-KIT, FLT3, and M-CSFR.  The two PDGF receptor subtypes show different affinities for the dimeric PDGF isoforms. PDGFRα binds to all ligands except PDGF-D, while PDGFRβ binds to PDGF-B and -D only. The two PDGF receptors contain five extracellular immunoglobulin-like domains, a single transmembrane domain, and an intracellular split kinase domain, which is formed by two lobes connected by a flexible polypeptide linker, the kinase insert. The binding of PDGFs to their receptors induces receptor dimerization. PDGF receptors are degraded after activation by a mechanism that involves ligand-induced endocytosis and degradation in lysosomes. The soluble form of PDGFRα was detected in conditioned media from human cell lines. The soluble receptor was derived by proteolytic clipping of the full length PDGFR. Soluble PDGFRβ inhibits hepatic stellate cell activation and attenuates liver fibrosis. Also, soluble PDGFRβ inhibits intraosseous growth of breast cancer cells in nude mice. PDGFRβ mediates recruitment of pericytes and vascular smooth muscle cells to newly formed vessels in response to PDGF-BB secreted by endothelial cells. PDGFRβ knockout embryos develop abnormal glomeruli in the kidney (developmental defect  of mesangial cells) capillary microaneurysm, cardiac and placental defects, and die at E16–E19 from hemorrhage. High levels of PDGFRβ expression have been associated with tumor recurrence in primary colorectal cancer, and it correlates with poor disease-free survival in pancreatic, colon, and ovarian cancer patients.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PDGFRβ Recombinant Protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding between recombinant human PDGF-BB (Cat. No. 577306) and recombinant human PDGFRβ (Cat. No. 767206). The ND50 range is 0.08 – 0.6 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814474 (BioLegend Cat. No. 622803)
AB_2814475 (BioLegend Cat. No. 622804)

Antigen Details

Structure
Homodimer
Distribution

Cells of mesenchymal origin, mesothelioma cell lines, pericytes and vascular smooth muscle cells.

Function
Proliferation, transformation, migration, survival, and apoptosis of mesenchymal cells, in development and pathogenesis. Development of hematopoietic/endothelial precursors, regulates craniofacial development.
Interaction
Interacts with a number of kinases (including Raf-1, NCK1, FAK, Fyn, others) as well as adaptor molecules and signaling intermediates (Grb2, Grb4, RasGAP, SHP2, SHC1, Crk, others). Has also been shown to associate with integrin β3 and nexin sorting.
Cell Type
Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Tiesman J and Hart CE. 1993. J. Biol. Chem. 13:9621-8.
  2. Lindahl P, et al. 1997. Science 277:242.
  3. Hart CE, et al. 1998. Science. 240:1529.
  4. Borkham-Kamphorst E, et al. 2004 Lab Invest. 84:766.
  5. Lennartsson J, et al. 2006. J. Biol. Chem. 281:39152.
  6. Van den Akker NM, et al. 2008 Dev. Dyn. 237: 494.
  7. Toffalini F, Demoulin JB. 2010. Blood 116:2429.
  8. Shan H, et al. 2011. Cancer Sci. 102:1904.
  9. Demoulin JB, Montano-Almendras CP. 2012. Am. J. Blood Res. 2:44.
  10. Heldin CH, Lennartsson J. 2013. Cold Spring Harb. Perspect. Biol. 5:a009100.
  11. Weissmueller S, et al. 2014 Cell. 157:382.
  12. Fantauzzo KA, Soriano P. 2016. Genes Dev. 30:2443. 
Gene ID
5159 View all products for this Gene ID
UniProt
View information about CD140b on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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