Ultra-LEAF™ Purified anti-human CD119 (IFN-γ R α chain) Antibody

Pricing & Availability
Clone
GIR-208 (See other available formats)
Workshop
VI C-110
Other Names
IFN-γR, IFN-γRα, IFN-α R1, CDw119
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Human peripheral blood lymphocytes stained with LEAF™ purified GIR-208, detected with biotinylated anti-mouse IgGs and Sav-PE
  • Human peripheral blood lymphocytes stained with LEAF™ purified GIR-208, detected with biotinylated anti-mouse IgGs and Sav-PE
Cat # Size Price Quantity Avail. Save
308609 100 µg £165
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308610 1 mg £413
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Description

CDw119 is a 90-100 kD type I transmembrane protein, also known as IFN-γ R α chain or IFN-γRI. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. The IFN-γ R α chain is a member of the class II cytokine receptor family. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IFN-γRα, Purified from human placenta
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1 under non-reducing conditions, immunoprecipitation1, immunohistochemistry3 of snap frozen sections, and blocking1 of IFN-γ binding to IFN-γ R α chain. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. Nos. 308609 & 308610).

Application References

(PubMed link indicates BioLegend citation)
  1. Sheehan K, et al. 1988. J. Immunol. 140:4231. (WB IP Block)
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Peyman JA, et al. 1992. J. Immunol. 149:2675. (IHC)

Antigen Details

Structure
Ig superfamily, class II cytokine receptor family, associates with IFN-γ receptor β subunit, 90-100 kD
Distribution

Macrophages, monocytes, B, T, and NK cells, neutrophils, endothelial and epithelial cells, fibroblasts

Function
Activates Jak1, Jak2, STAT1, host defense
Ligand/Receptor
IFN-γ
Cell Type
B cells, Endothelial cells, Epithelial cells, Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Calderon J, et al. 1988. P. Natl. Acad. Sci. USA 85:4837.
2. Basler C, et al. 2002. Int. Rev. Immunol. 21:305.
3. Brierley M, et al. 2002. J. Interferon Cytokine Res. 22:835.

Gene ID
3459 View all products for this Gene ID
UniProt
View information about CD119 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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