Ultra-LEAF™ Purified anti-human CCL4 Antibody

Pricing & Availability
Clone
W15138B (See other available formats)
Other Names
C-C motif chemokine 4, Immune activation protein 2 (ACT-2), Macrophage inflammatory protein 1-beta, MIP-1-beta, Protein H400, SIS-gamma, Small-inducible cytokine A4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W15138B_ULEAF_CCL4_Antibody_1_090319
Recombinant human CCL4 (Black Circles, Cat. No. 554706) induces the chemotaxis of mouse Baf3-hCCR5 transfectants chemoattractant cells. Anti-human CCL4 antibody (Clone W15138B, Purple Squares) neutralizes the chemotaxis of recombinant human CCL4 at 2 ng/mL in Baf3-hCCR5 cells in a dose-dependent manner while the isotype control (Grey Triangles, Cat. No. 400562) does not have the effect. ND50 range: 3 - 30 ng/mL.
  • W15138B_ULEAF_CCL4_Antibody_1_090319
    Recombinant human CCL4 (Black Circles, Cat. No. 554706) induces the chemotaxis of mouse Baf3-hCCR5 transfectants chemoattractant cells. Anti-human CCL4 antibody (Clone W15138B, Purple Squares) neutralizes the chemotaxis of recombinant human CCL4 at 2 ng/mL in Baf3-hCCR5 cells in a dose-dependent manner while the isotype control (Grey Triangles, Cat. No. 400562) does not have the effect. ND50 range: 3 - 30 ng/mL.
Cat # Size Price Quantity Avail. Save
625403 100 µg £165
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625404 1 mg £437
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Description

CCL4 is an important proinflammatory cytokine involved in acute and chronic inflammatory responses. CCL4 attracts monocytes, NK cells, dendritic cells, and lymphocytes to sites of injury and inflammation. CCL4 signals through the G-protein-coupled receptor CCR5, and since CCR5 is a major co-receptor for M-tropic HIV strains, the anti-HIV effect of CCL4 has been explored. Thus, binding of CCL4 to CCR5 inhibits HIV entry and reduces the cell surface expression of CCR5. CCL4 has been identified as one of the major HIV-suppressive factors produced by CD8+ T cells. CCL4 also protects against type I diabetes by suppressing islet beta-cell inflammatory responses. In a rat model of experimental autoimmune encephalomyelitis, anti-CCL4 treatment using a neutralizing antibody significantly delayed the development, and shortened the duration, of the disease. Activated human peripheral lymphocytes secrete a truncated form of CCL4 that lacks the first two amino acids. CCR5 and truncated CCL4 bind to CCR1 and CCR2. Like CCL3, CCL4 is highly acidic and can form high-molecular weight polymers. Consistent with this observation, CCL4 is secreted as high molecular weight aggregates from HIV-I specific cytotoxic T lymphocytes. The polymerization of CCL4 is reversible and is important for in vivo activity. Heterodimers of CCL3 and CCL4 have also been described.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Human CCL4
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CCL4 (2 ng/mL) on mouse Baf3-hCCR5 transfectants chemoattractant cells. ND50 range: 3 - 30 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814486 (BioLegend Cat. No. 625403)
AB_2814487 (BioLegend Cat. No. 625404)

Antigen Details

Structure
Chemokine
Distribution

CCL4 is secreted by many mature hematopoietic cells including macrophages, T and B lymphocytes, neutrophils, dendritic cells, mast cells and NK cells. Influenza virus-infected bronchial and nasal epithelial cells produce CCL4.

Function
CCL4 attracts monocytes, NK cells, dendritic cells, and T cells. CCL4 acts as an HIV-suppressive factor. Activating transcription factor 3 (ATF3) limits the amount of CCL4 released by macrophages. IFN-γ and NOS2 repress CCL3 and CCL4 in mouse macrophages via ATF3.
Interaction
Monocytes, NK cells, dendritic cells, and lymphocytes.
Ligand/Receptor
CCR5
Cell Type
Dendritic cells, Endothelial cells, Leukocytes, Lymphocytes, Macrophages, Monocytes, NK cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Raport CJ, et al. 1996. J. Biol. Chem. 271:17161.
  2. Guan E, et al. 2001. J. Biol. Chem. 276:12404.
  3. Guan E, et al. 2002. J. Biol. Chem. 277:32348.
  4. Meagher C, et al. 2007. Diabetes. 56:809.
  5. Maurer M and von Stebut E, 2004. Int. J. Biochem. Cell. Biol. 36:1882-6.
  6. Cocchi F, et al. 1995. Science 270:1811.
  7. Ren M, et al. 2010. EMBO J. 29:3952.
  8. Kamin-Lewis R, et al. 2001. Proc. Natl. Acad. Sci. USA 98:9283.
  9. Chandrasekar B, et al. 2013. J. Infect. Dis. 207:1556.
Gene ID
6351 View all products for this Gene ID
UniProt
View information about CCL4 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09/03/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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