Ultra-LEAF™ Purified anti-human CCL27 (CTACK) Antibody

Pricing & Availability
Clone
A15094F (See other available formats)
Regulatory Status
RUO
Other Names
Cutaneous T cell-attracting chemokine (CTACK), embrional stem cell chemokine (ESKINE), IL11RA-Locus chemokine interleukin-11 receptor a-locus chemokine (ILC), amin terminal peptide sequence (ALP)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A15094F_ULEAF_CCL27_CTACK_Antibody_072821.png
Recombinant human CCL27 (CTACK) (Cat. No. 583602) (black circles) induces the chemotaxis of mouse Baf3-hCCR10 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-human CCL27 (CTACK) antibody (clone A15094F) (purple squares) neutralizes the chemotaxis of recombinant human CCL27 (CTACK) at 50 ng/mL on mouse Baf3- hCCR10 cells in a dose-dependent manner whereas the GoInVivo™ purified mouse IgG2b, κ isotype ctrl antibody (Cat. No. 400366) (gray triangles) does not have the effect. ND50 range: 0.05 - 0.8 µg/mL.
  • A15094F_ULEAF_CCL27_CTACK_Antibody_072821.png
    Recombinant human CCL27 (CTACK) (Cat. No. 583602) (black circles) induces the chemotaxis of mouse Baf3-hCCR10 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-human CCL27 (CTACK) antibody (clone A15094F) (purple squares) neutralizes the chemotaxis of recombinant human CCL27 (CTACK) at 50 ng/mL on mouse Baf3- hCCR10 cells in a dose-dependent manner whereas the GoInVivo™ purified mouse IgG2b, κ isotype ctrl antibody (Cat. No. 400366) (gray triangles) does not have the effect. ND50 range: 0.05 - 0.8 µg/mL.
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940303 100 µg £171.00
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940304 1 mg £448.00
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Description

CCL27 is highly expressed in skin where it is constitutively produced by keratinocytes. CCL27 binds to CCR10 (shared with CCL28) which is expressed on melanocytes, fibroblasts, and dermal microvascular endothelial cells. The interaction of CCL27 with CCR10 has a main role in skin homeostasis and inflammation. It has been described that more than 90% of the lymphocytes in the skin of patients with psoriasis, atopic dermatitis, and allergic-contact dermatitis express CCR10. In melanoma, CCR10 increases the ability of neoplastic cells to grow, invade tissues, disseminate to lymph nodes, and escape host immune responses. Also, it has been described that human skin tumors elude T cell-mediated antitumor immune responses by down-regulating the expression of CCL27. Interestingly, this down-regulation is mediated by EGFR signaling. In addition, CCL27 upregulation in melanoma cells impairs tumor growth due to the recruitment and accumulation of T cells and natural killer cells at tumor sites, resulting in enhanced antitumor immune responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CCL27 (CTACK)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CCL27 (CTACK) (50 ng/mL) on mouse Baf3-hCCR10 transfectant chemoattractant cells. ND50 range: 0.05 - 0.8 µg /mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2894526 (BioLegend Cat. No. 940303)
AB_2894526 (BioLegend Cat. No. 940304)

Antigen Details

Structure
Chemokine
Distribution

CCL27 is expressed in gonads, thymus, placenta and skin. CCL27 is expressed by keratinocytes, fibroblasts.

Function
CCL27 attracts CLA+ memory T cells to the skin. CCL27 mRNA is induced by TNF-α and IL-1 in keratinocytes, and this increase is down-regulated by IL-10. CCL27 expression is down-regulated in keratinocytes by EGF. CCL27 has important function in T cell-mediated skin inflammation, antitumor immune responses and neuroimmune communication with T cells during peripheral allergic inflammation.
Interaction
CLA+ memory T cells, melanocytes, dermal fibroblasts, dermal microvascular endothelial cells, skin-derived Langerhans cells, and plasmacytoid dendritic cells
Ligand/Receptor
CCR10
Cell Type
Astrocytes, Epithelial cells, Fibroblasts
Biology Area
Adaptive Immunity, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Morales J, et al. 1999. P Natl Acad Sci USA. 96:14470.
  2. Homey B, et al. 2000. J Immunol. 164:3465.
  3. Homey B, et al. 2002. Nature Med. 8:157.
  4. Okada N, et al. 2006. Cancer Gene Ther. 13:393.
  5. Pivarcsi A, et al. 2007. P Natl Acad Sci USA. 104:19055.
  6. Kai H, et al. 2011. Pathol Res Pract. 207:43.
Gene ID
10850 View all products for this Gene ID
UniProt
View information about CCL27 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07/28/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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