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Ultra-LEAF™ Purified anti-human BDNF Recombinant Antibody

Pricing & Availability
Clone
QA19A71 (See other available formats)
Regulatory Status
RUO
Other Names
Brain-derived neurotrophic factor, ANON2, BLUN2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA19A71_ULEAF_BDNF_Recombinant_Antibody_040921.png
Recombinant human BDNF (black circles) (Cat. No. 788904) induces the proliferation of C6 glioma cells. Ultra-LEAF™ purified anti-human BDNF recombinant antibody (purple squares) (clone QA19A71) inhibits the effect of recombinant human BDNF (at 4 µg/mL) in C6 cells in a dose - dependent manner whereas the isotype control (gray triangles) (Cat. No. 400193) does not have the effect. The ND50 = 1 - 5 µg/mL.
  • QA19A71_ULEAF_BDNF_Recombinant_Antibody_040921.png
    Recombinant human BDNF (black circles) (Cat. No. 788904) induces the proliferation of C6 glioma cells. Ultra-LEAF™ purified anti-human BDNF recombinant antibody (purple squares) (clone QA19A71) inhibits the effect of recombinant human BDNF (at 4 µg/mL) in C6 cells in a dose - dependent manner whereas the isotype control (gray triangles) (Cat. No. 400193) does not have the effect. The ND50 = 1 - 5 µg/mL.
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936803 100 µg £185
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936804 1 mg £490
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Description

Brain-derived neurotrophic factor (BDNF) is the second member of the NGF family of neurotrophic factors (neurotrophins) that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5). BDNF shares approximately 55% amino acid identity with other family members. The amino acid sequence of mature BDNF is identical in all mammals examined. All neurotrophins are non-covalently linked homodimers and initially produced as proneurotrophins. These proneurotrophins have distinct biological activity from their mature counterparts. Mature BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems during development. BDNF participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. BDNF is also a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant human BDNF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by the inhibition of proliferation induced by human BDNF (4 µg/mL) on C6 glioma cells. ND50 range: 1 - 5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2892499 (BioLegend Cat. No. 936803)
AB_2892499 (BioLegend Cat. No. 936804)

Antigen Details

Structure
Non-covalently linked homodimer
Distribution

Ubiquitous with high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis.

Function
Supports the survival of sensory and sympathetic neurons, and also dopaminergic midbrain neurons.
Interaction
Sensory neurons, sympathetic neurons, midbrain dopaminergic neurons.
Ligand/Receptor
TrkB
Cell Type
Neural Stem Cells, Neurons
Biology Area
Neurodegeneration, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Growth Factors
Antigen References
  1. Baloh RH, et al. 1998. Neuron. 21:1291-302.
  2. Masure S, et al. 1999. Eur J Biochem. 266:892-902.
  3. Gardell LR, et al. 2003. Nature Med. 9:1383-9.
  4. Honma Y, et al. 2002. Neuron. 35:267-82.
  5. Banerjee A, et al. 2012. PLoS One. 7:e50098.
  6. Wong LE, et al. 2015. Proc Natl Acad Sci USA. 112:6170-5.
  7. Zhang M, et al. 2016. Oncotarget. 7:3267-82.
Gene ID
627 View all products for this Gene ID
UniProt
View information about BDNF on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 04/09/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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