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Ultra-LEAF™ Purified anti-GDNF Antibody

Pricing & Availability
Clone
A16099G (See other available formats)
Regulatory Status
RUO
Other Names
Glial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor (ATF), ATF1, ATF2, HSCR3, HFB1-GDNF
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16099G_ULEAF_GDNF_Antibody_1_121319
Recombinant human GFRα1 (black circles) binds to immobilized recombinant human GDNF (Cat. No. 760402). Anti-human GDNF antibody (purple squares, clone A16099G) inhibits the binding in a dose-dependent manner while the isotype control (grey triangles, Cat. No. 401215) does not have the effect. This antibody blocks the binding of 0.1 µg/mL recombinant human GFRα1 protein to 0.5 µg/mL of immobilized recombinant human GDNF with an ND50 = 0.05 - 0.4 µg/mL.
  • A16099G_ULEAF_GDNF_Antibody_1_121319
    Recombinant human GFRα1 (black circles) binds to immobilized recombinant human GDNF (Cat. No. 760402). Anti-human GDNF antibody (purple squares, clone A16099G) inhibits the binding in a dose-dependent manner while the isotype control (grey triangles, Cat. No. 401215) does not have the effect. This antibody blocks the binding of 0.1 µg/mL recombinant human GFRα1 protein to 0.5 µg/mL of immobilized recombinant human GDNF with an ND50 = 0.05 - 0.4 µg/mL.
Cat # Size Price Quantity Check Availability Save
670903 100 µg £185
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670904 1 mg £490
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Description

Glial cell-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily and was first identified as a growth factor supporting the survival of embryonic midbrain neurons. GDNF has been shown to promote the development, differentiation, and survival of dopaminergic neurons by signaling through GDNF family receptor α-1 (GFRα1) and the coreceptor RET receptor tyrosine kinase. Dysregulated expression of GDNF has been reported to be associated with several mental diseases, which include depression and schizophrenia. GDNF is considered as a promising therapeutic agent for the treatment of Parkinson's disease because it can protect dopaminergic neurons from neurotoxins and promote neuron regeneration after injury. In addition to the functions in the nervous system, GDNF is also required for the differentiation of spermatogonia and kidney development. Upregulated expression of GDNF was observed in various cancer cell lines and correlates with malignant cancer progression, suggesting a role of GDNF in carcinogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human GDNF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding between recombinant human GFRα1 and recombinant human GDNF (Cat. No. 760402). The ND50 range is 0.05 - 0.4 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2820112 (BioLegend Cat. No. 670903)
AB_2820113 (BioLegend Cat. No. 670904)

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

GDNF is a secreted protein that is widely expressed in the developing and adult central nervous system.

Function
GDNF is a neurotrophic factor that promotes the development, differentiation, and the survival of dopaminergic neurons. It also participates in the recovery process after brain surgery.
Interaction
GDNF targets the neuronal cells expressing GDNF family receptors.
Ligand/Receptor
GDNF interacts with GDNF family receptor α-1 (GFRα1) and has a lower affinity with GFRα2, GFRα3, and GFRα4.
Cell Type
Embryonic Stem Cells, Mature Neurons, Neural Stem Cells, Neurons, Schwann Cells
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Growth Factors
Antigen References
  1. Allen SJ, et al. 2013. Pharmacol Ther. 138:155.
  2. Hendrich J, et al. 2012. Neuroscience. 219:204.
  3. Hidalgo-Figueroa M, et al. 2012. J Neurosci. 32:864.
  4. Pascual A, et al. 2011. J Mol Endocrinol. 3:R83-92.
  5. Carnicella S, et al. 2009. Pharmacol Ther. 122:9.
  6. Sariola H, et al. 2003. J Cell Sci. 116:3855.
Gene ID
2668 View all products for this Gene ID
UniProt
View information about GDNF on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 06/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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