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Ultra-LEAF™ Purified anti-CD325 (N-Cadherin) Antibody

Pricing & Availability
Clone
13A9 (See other available formats)
Regulatory Status
RUO
Other Names
CDHN, CDw325, NCAD, N-cadherin 1, Cadherin 2, N-cadherin (neuronal), cadherin-2, calcium-dependent adhesion protein, neuronal, neural cadherin, Neural-cadherin
Isotype
Mouse IgG1, κ
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Product Citations
publications
13A9_PURE_CD325_Antibody_WB_081018
Western blot of Ultra-LEAF™ Purified anti-CD325 (N-Cadherin) antibody (clone 13A9). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain membrane lysate; Lane 3: 10 µg of mouse brain membrane lysate; Lane 4: 10 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 13A9_PURE_CD325_Antibody_WB_081018
    Western blot of Ultra-LEAF™ Purified anti-CD325 (N-Cadherin) antibody (clone 13A9). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain membrane lysate; Lane 3: 10 µg of mouse brain membrane lysate; Lane 4: 10 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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844703 100 µg £110
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Description

CD325 (N-cadherin) is important for neuronal migration during development, neurite outgrowth and target finding during differentiation, initial formation of synapses, and synaptic plasticity in mature neurons. CD325 (N-cadherin) is a 130 kD single pass transmembrane protein. Its extracellular region consists of five extracellular cadherin domains and has one cytoplasmic domain. N-cadherin is involved in organogenesis and in the maintenance of organ architecture by contributing to the sorting of heterogeneous cell types and in the cell adhesion needed to form tissues. N-cadherin is expressed by stem cells, myeloblasts, endothelial cells, fibroblasts, and also is expressed in neural and muscle tissues along with some types of carcinoma cells. CD325 associates with the cytoskeleton trough catenin proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human N-cadherin cytoplasmic domain fused to recombinant MBP.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunfluorescence microscopy1,2,3,6, immunohistochemical staining4, and immunoprecipitation2,5,7.

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 844703) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Wahl JK, et al. 2003. J. Biol. Chem. 278:17269. (WB, IF)
  2. Knudsen KA, et al. 1995. J. Cell Biol. 130:67. (WB, IF, IP)
  3. Machell NH, et al. 2000. Biol. Reprod. 63:797. (WB, IF)
  4. Han AC, et al. 1997. Hum. Pathol. 28:641. (IHC)
  5. Van Aken EH, et al. 2002. Invest Ophthalmol. Vis. Sci. 43:595. (WB, IP)
  6. Tian G, et al. 2009. J. Biol. Chem. 284:18980. (IF)
  7. Nieman MT. 1999. J. Cell Biol. 147:631. (IP, WB)
RRID
AB_2565807 (BioLegend Cat. No. 844703)

Antigen Details

Structure
Member of cadherin family, single pass transmembrane protein, extracellular region consists of five extracellular cadherin domains, one cytoplasmic domain, 130 kD.
Distribution

Cell membrane, stem cells, myeloblasts, endothelial cells, fibroblasts, neural and muscle tissues, and some types of carcinoma cells.

Function
Embryonic development, maintain tissue architecture, cell sorting and cell adhesion in tissues, promote motility, cell junction organization, pre and post synaptic adhesion, and ERK signaling.
Interaction
Catenins.
Cell Type
Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hirano S, et al. 2012. Physiol. Rev. 92:597.

Gene ID
1000 View all products for this Gene ID
UniProt
View information about CD325 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 3    Revision Date: 06/15/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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