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Ultra-LEAF™ Purified anti-β Catenin 1 (CTNNB1) Antibody

Pricing & Availability
Clone
12F7 (See other available formats)
Regulatory Status
RUO
Other Names
Catenin (Cadherin-Associated Protein), Beta 1, 88 kD, CTNNB1, TNNB, MRD19, armadillo, catenin beta-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
12F7_PURE_Catenin1_Antibody_WB_070815
Western blot was incubated with either clone 12F7 (lanes 2-3) or mouse IgG1 isotype control (lanes 5-6) at 10 µg/ml. Lanes 1&4: Molecular weight markers. Lanes 2&5: Mouse whole brain lysate. Lanes 3&6: Rat whole brain lysate.
  • 12F7_PURE_Catenin1_Antibody_WB_070815
    Western blot was incubated with either clone 12F7 (lanes 2-3) or mouse IgG1 isotype control (lanes 5-6) at 10 µg/ml. Lanes 1&4: Molecular weight markers. Lanes 2&5: Mouse whole brain lysate. Lanes 3&6: Rat whole brain lysate.
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844603 100 µg £223
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Description

β-catenin is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. β-catenin also plays a key role in Wnt signaling pathways and thus is involved in neural differentiation, synaptic plasticity, neurodegenerative disease, and prevention of apoptosis. Finally, this protein binds to the product of the adenomatous polyposis coli (APC) gene, which is mutated in the adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant β-catenin (N-terminal fragment) fused to MBP.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunfluorescence microscopy4 and immunohistochemical staining3.

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 844603) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Sacco P, et al. 1995. J. Biol. Chem. 270:20201. (WB)
  2. Johnson KR, et al. 1993. Exp. Cell Res. 207:252.
  3. Gupta K, et al. 2012. J. Ped. Hem. Onc. 34:320. (IHC-P)
  4. Radice G, et al. 1997. Dev. Bio. 181:64. (IHC-P)
Product Citations
  1. Xie S, et al. 2020. Ann Transl Med. 1.384027778. PubMed
RRID
AB_2565805 (BioLegend Cat. No. 844603)

Antigen Details

Structure
88 kD armadillo repeat containing protein with an unstructured N and C terminal.
Distribution

β-catenin localizes to cell-cell contacts (adherens junctions) and the cell membrane as well as the cytoplasmic pool of protein. β-catenin can also translocate to the nucleus and is in interphase found in puncta at the proximal end of centrioles. In mitosis, it localizes to the centrosomes at the spindle.

Function
Wnt signaling pathway, regulation of cell-cell adhesion, neurogenesis, transcription, transcription regulation, early embryonic patterning, asymmetric cell division, stem cell renewal, and the epithelial to mesenchymal transition.
Interaction
Wnt signaling pathway, apoptotic cleavage of cellular proteins, and EphB-EphrinB signaling.
Biology Area
Cell Adhesion, Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Adhesion Molecules
Antigen References

1. Hirano S, et al. 2012. Physiol. Rev. 92:597.

Gene ID
1499 View all products for this Gene ID
UniProt
View information about beta Catenin 1 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07/15/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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