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TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody

Pricing & Availability
Clone
S17011E (See other available formats)
Regulatory Status
RUO
Other Names
CD16, CD32
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
TruStain_FcX_Plus_CD16-CD32_Antibody_122018_updated.png
BALB/c splenocytes were incubated with TruStain FcX™ PLUS (clone S17011E, 0.25µg/106 cells, filled histogram) or TruStain FcX™ (clone 93, 1µg/106 cells, red line histogram) to block the Fc receptors, or were left untreated (black line histogram); then stained with CD90.2 (Thy-1.2, clone 53-2.1) FITC. Note the high background in the untreated cells and the bigger reduction in the background staining when the cells were incubated with TruStain FcX™ Plus compared to TruStain FcX™.
  • TruStain_FcX_Plus_CD16-CD32_Antibody_122018_updated.png
    BALB/c splenocytes were incubated with TruStain FcX™ PLUS (clone S17011E, 0.25µg/106 cells, filled histogram) or TruStain FcX™ (clone 93, 1µg/106 cells, red line histogram) to block the Fc receptors, or were left untreated (black line histogram); then stained with CD90.2 (Thy-1.2, clone 53-2.1) FITC. Note the high background in the untreated cells and the bigger reduction in the background staining when the cells were incubated with TruStain FcX™ Plus compared to TruStain FcX™.
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156603 50 µg £45
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156604 500 µg £189
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Description

CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ PLUS is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors and is more effective than TruStain FcX™.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Purity
The antibody was purified by affinity chromatography.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Concentration
0.5 mg/ml
Storage & Handling
The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain FcX™ PLUS for 5-10 minutes, on ice, at 0.25 µg per 106 cells in a volume of 100 µl, prior to immunostaining. It is not necessary to wash the cells between the blocking and immunostaining steps.

Application Notes

Clone S17011E blocks both clone 93 and 2.4G2 also raised against mouse CD16/32

Product Citations
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  16. Ikeda S, et al. 2022. Sci Rep. 12:11564. PubMed
  17. Thinard R, et al. 2022. Pharmaceutics. 14:. PubMed
  18. So EY, et al. 2021. Am J Physiol Cell Physiol. 321:C569. PubMed
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  22. Dong L, et al. 2022. Cells. 11:. PubMed
  23. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
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  26. Guo S, et al. 2021. Sci Rep. 11:23745. PubMed
  27. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  28. Galani IE, et al. 2022. STAR Protoc. 3:101151. PubMed
  29. Olson B, et al. 2021. Brain Behav Immun. 97:102. PubMed
  30. Grubiši? V, et al. 2020. Cell Rep. 32:108100. PubMed
  31. Montel-Hagen A, et al. 2022. Curr Protoc. 2:e403. PubMed
  32. Chen AF, et al. 2022. Nat Methods. 19:547. PubMed
  33. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  34. Kos S, et al. 2019. PLoS One. 14:e0217762. PubMed
  35. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  36. Chen S, et al. 2022. iScience. 25:105176. PubMed
  37. Oya Y, et al. 2021. Front Immunol. 12:779709. PubMed
  38. Hobbs BE, et al. 2021. Pathogens. 10:. PubMed
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  41. Phan TS, et al. 2021. Sci Adv. 7:. PubMed
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  52. Xu L, et al. 2022. Front Immunol. 13:841141. PubMed
  53. Matsuda A, et al. 2020. J Appl Toxicol. 40:706. PubMed
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  55. Badgujar DC, et al. 2020. PLoS Pathog. 16:e1009016. PubMed
RRID
AB_2783137 (BioLegend Cat. No. 156603)
AB_2783138 (BioLegend Cat. No. 156604)

Antigen Details

Structure
Ig superfamily, 40-60 kD
Distribution

B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells

Ligand/Receptor
IgG
Cell Type
B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14130 View all products for this Gene ID 14131 View all products for this Gene ID
UniProt
View information about CD16/32 on UniProt.org
Go To Top Version: 2    Revision Date: 01/22/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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