True-Phos™ Perm Buffer

Pricing & Availability
Other Names
Phospho-protein Fixation/Permeabilization Buffer Set
Ave. Rating
1 reviews
Product Citations
publications
True_Phos_Perm_Buffer_050616
Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) IL-4 for 15 minutes, fixed with Fixation Buffer, permeabilized with cold True-Phos™ Perm Buffer, and intracellularly stained with anti-STAT6 Phospho (Tyr641) (clone A15137E) PE.
  • True_Phos_Perm_Buffer_050616
    Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) IL-4 for 15 minutes, fixed with Fixation Buffer, permeabilized with cold True-Phos™ Perm Buffer, and intracellularly stained with anti-STAT6 Phospho (Tyr641) (clone A15137E) PE.
Cat # Size Price Quantity Avail. Save
425401 125 ml £91
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Description

True-Phos™ Perm Buffer has been developed to permeabilize fixed peripheral blood mononuclear cells (PBMCs), lysed whole blood, cell lines, or splenocytes. Cells can be stimulated cytokines (e.g. IL-4, IL-6, etc.), antibodies (e.g. anti-CD3, clone HIT3a), or phorbol-12-myristate 13-acetate and ionomycin (Cell Activation Cocktail; Cat. No. 423301), and fixed with Fixation Buffer (Cat. No. 420801), or for whole blood, RBC Lysis/Fixation Solution (Cat. No. 422401). This buffer has been designed for use with antibodies for phospho-proteins (e.g. p-STAT6, p-ERK, etc.) and other intracellular targets (e.g. Cyclin B1, pan-STAT3, etc.). Not all cell surface markers are compatible with BioLegend’s True-Phos™ Perm Buffer.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
This buffer is guaranteed for 3 months. Store between 2°C and 8°C, protected from prolonged exposure to light.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this product is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Use 1 mL of True-Phos™ Perm Buffer per 1 million cells, or 100 µL of whole blood.

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells and to study phosphorylation kinetics in our Scientific Poster Library.

Product Citations
  1. Ryan C, et al. 2016. Blood. 128(25):2899-2908. PubMed
Publication Library

Antigen Details

Biology Area
Cell Biology, Signal Transduction, Transcription Factors
Molecular Family
Phospho-Proteins
Gene ID
NA

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Go To Top Version: 2    Revision Date: 06/15/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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