TotalSeq™-D0218 anti-human CD62P (P-Selectin) Antibody

Pricing & Availability
AK4 (See other available formats)
Regulatory Status
VI P-44
Other Names
Mouse IgG1, κ
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304951 10 µg £253
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CD62P is a 140 kD type I transmembrane glycoprotein also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Reported Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4 and in vitro blocking of adhesion of platelets1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304947 & 304948).

Additional Product Notes

TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit

The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Application Technical Notes.

Application References

(PubMed link indicates BioLegend citation)
  1. Skinner M, et al. 1991. J. Biol. Chem. 266:5371. (Block)
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Yen YT, et al. 2006. J. Virol. 80:2684.
  4. Sato Y, et al. 2005. Blood 106:428. (IHC)
AB_2892359 (BioLegend Cat. No. 304951)

Antigen Details

Selectin, type I glycoprotein, 140 kD

Activated platelets, megakaryocytes, endothelial cells

Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions
CD162 (PSGL-1), CD24 and sialylated Lewis X
Cell Type
Endothelial cells, Megakaryocytes, Neutrophils, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. McEver R, et al. 1995. J. Biol. Chem. 270:11025.
2. Varki A. 1994. P. Natl. Acad. Sci. USA 91:7390.

Gene ID
6403 View all products for this Gene ID
View information about CD62P on

Related FAQs

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Other Formats

View All CD62P Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD62P (P-Selectin) AK4 FC
FITC anti-human CD62P (P-Selectin) AK4 FC
PE anti-human CD62P (P-Selectin) AK4 FC
PE/Cyanine5 anti-human CD62P (P-Selectin) AK4 FC
Purified anti-human CD62P (P-Selectin) AK4 FC,IHC-F
Biotin anti-human CD62P (P-Selectin) AK4 FC
Alexa Fluor® 488 anti-human CD62P (P-Selectin) AK4 FC
Alexa Fluor® 647 anti-human CD62P (P-Selectin) AK4 FC
Brilliant Violet 605™ anti-human CD62P (P-Selectin) AK4 FC
Brilliant Violet 421™ anti-human CD62P (P-Selectin) AK4 FC
PE/Cyanine7 anti-human CD62P (P-Selectin) AK4 FC
PerCP/Cyanine5.5 anti-human CD62P (P-Selectin) AK4 FC
Alexa Fluor® 700 anti-human CD62P (P-Selectin) AK4 FC
PE/Dazzle™ 594 anti-human CD62P (P-Selectin) AK4 FC
APC/Fire™ 750 anti-human CD62P (P-Selectin) AK4 FC
TotalSeq™-A0218 anti-human CD62P (P-Selectin) AK4 PG
Brilliant Violet 650™ anti-human CD62P (P-Selectin) AK4 FC
Brilliant Violet 510™ anti-human CD62P (P-Selectin) AK4 FC
Brilliant Violet 711™ anti-human CD62P (P-Selectin) AK4 FC
APC/Cyanine7 anti-human CD62P (P-Selectin) AK4 FC
Brilliant Violet 785™ anti-human CD62P (P-Selectin) AK4 FC
TotalSeq™-C0218 anti-human CD62P (P-Selectin) AK4 PG
Ultra-LEAF™ Purified anti-human CD62P (P-Selectin) AK4 FC
TotalSeq™-B0218 anti-human CD62P (P-Selectin) Antibody AK4 PG
TotalSeq™-D0218 anti-human CD62P (P-Selectin) AK4 PG
PE anti-human CD62P AK4 FC
FITC anti-human CD62P AK4 FC
Go To Top Version: 1    Revision Date: 05/24/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD62P (P-Selectin)

  • FITC anti-human CD62P (P-Selectin)

  • PE anti-human CD62P (P-Selectin)

  • PE/Cyanine5 anti-human CD62P (P-Selectin)

  • Purified anti-human CD62P (P-Selectin)

  • Biotin anti-human CD62P (P-Selectin)

  • Alexa Fluor® 488 anti-human CD62P (P-Selectin)

  • Alexa Fluor® 647 anti-human CD62P (P-Selectin)

  • Brilliant Violet 605™ anti-human CD62P (P-Selectin)

  • Brilliant Violet 421™ anti-human CD62P (P-Selectin)

  • PE/Cyanine7 anti-human CD62P (P-Selectin)

  • PerCP/Cyanine5.5 anti-human CD62P (P-Selectin)

  • Alexa Fluor® 700 anti-human CD62P (P-Selectin)

  • PE/Dazzle™ 594 anti-human CD62P (P-Selectin)

  • APC/Fire™ 750 anti-human CD62P (P-Selectin)

  • TotalSeq™-A0218 anti-human CD62P (P-Selectin)

  • Brilliant Violet 650™ anti-human CD62P (P-Selectin)

  • Brilliant Violet 510™ anti-human CD62P (P-Selectin)

  • Brilliant Violet 711™ anti-human CD62P (P-Selectin)

  • APC/Cyanine7 anti-human CD62P (P-Selectin)

  • Brilliant Violet 785™ anti-human CD62P (P-Selectin)

  • TotalSeq™-C0218 anti-human CD62P (P-Selectin)

  • Ultra-LEAF™ Purified anti-human CD62P (P-Selectin)

  • TotalSeq™-B0218 anti-human CD62P (P-Selectin) Antibody

  • TotalSeq™-D0218 anti-human CD62P (P-Selectin)

  • PE anti-human CD62P

  • FITC anti-human CD62P


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