- FA-11 (See other available formats)
- Other Names
- Rat IgG2a
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- Product Citations
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Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.Product Details
- Antibody Type
- Host Species
- Purified Con A receptor glycoproteins from the P815 cell line
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
- The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer under optimal conditions. The solution is free of unconjugated DNA and unconjugated antibody.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
PG - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. For Proteogenomics TotalSeq™-A analysis, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
To maximize performance, centrifuge the antibody dilution (1.0 µg of antibody in 100 µl of staining buffer for every 1 million cells) before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
- Application Notes
Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, and immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3.
- Additional Product Notes
TotalSeq™ reagents are designed to profile protein expression at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The TotalSeq™-A barcode sequence associated with clone FA-11 is CTTTCTTTCACGGGA.
The flanking sequences are CCTTGGCACCCGAGAATTCCA, and the capture sequence, BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A, B represents either C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
The full oligomer sequence for this product, with the specific barcode in brackets is CCTTGGCACCCGAGAATTCCA [CTTTCTTTCACGGGA]BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A.
(PubMed link indicates BioLegend citation)
- Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
- Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
- Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
- Kayama H, et al. 2012. PNAS. 109:5010. PubMed
- Park S, et al. 2013. Biomaterials. 34:598. PubMed
- Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
- McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
- Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
AB_2783099 (BioLegend Cat. No. 137031)
- A member of the lysosomal-associated membrane protein (lamp) family.
Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.
- Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
- Cell Type
- Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Adhesion Molecules, CD Molecules, Innate Immune Signaling
- Antigen References
1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.
- Gene ID
- 12514 View all products for this Gene ID
- View information about CD68 on UniProt.org
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