TotalSeq™-A0448 anti-mouse CD204 (Msr1) Antibody

Pricing & Availability
1F8C33 (See other available formats)
Other Names
Msr1, MSR, SR-A, Scavenger receptor type A, Macrophage scavenger receptor I, Scara1
Rat IgG2a
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154703 10 µg £218
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CD204, also known as scavenger receptor A (SR-A) and the macrophage scavenger receptor (MSR), is 220 kDa, trimeric type II transmembrane protein, with one scavenger receptor cysteine-rich domain (SRCR). It is a phagocytic pattern-recognition receptor (PRR) expressed on macrophages and dendritic cells. CD204 is a receptor mediating recognition and internalization of low-density lipoprotein (LDL) by macrophages and plays a critical role in atherogenesis. CD204 also recognizes apoptotic cells, modified lipid proteins, and exogenous pathogen-associated molecular patterns (PAMPs), which results in the induction of innate immune and inflammatory responses. CD204 can act as a co-receptor for Toll-like receptors, such as TLR3, TLR4, or TLR9, to facilitate the expression of proinflammatory cytokines. CD204 has been implicated in several pathological processes such as Alzheimer’s disease, sepsis, ischemic injury, and coronary artery disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
Recombinant mouse CD204 extracellular domain
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer under optimal conditions. The solution is free of unconjugated DNA and unconjugated antibody.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. For Proteogenomics TotalSeq™-A analysis, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

To maximize performance, centrifuge the antibody dilution (1.0 µg of antibody in 100 µl of staining buffer for every 1 million cells) before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes

This clone has minimal recognition of CD204 in C57BL/6.

Additional Product Notes

TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g Illumina analyzers) are required. Please contact technical support for more information, or visit

The TotalSeq™-A barcode sequence associated with clone 1F8C33 is AGCTAGACACGTTGT.

The flanking sequences are CCTTGGCACCCGAGAATTCCA, and the poly A tail, BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A, where B represents either C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

The full oligomer sequence for this product, with the specific barcode in brackets is CCTTGGCACCCGAGAATTCCA [AGCTAGACACGTTGT]BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A.

AB_2783126 (BioLegend Cat. No. 154703)

Antigen Details

Trimeric type II transmembrane protein, one scavenger receptor cysteine-rich domain (SRCR), 220 kD.

Macrophages, dendritic cells.

Uptake of negatively charged molecules, role in pathology of atherosclerosis and Alzheimer’s disease.
Ligand Receptor
Acetylated and oxidized lipoproteins
Cell Type
Dendritic cells, Macrophages
Antigen References
  1. Shichita T, et al. 2017. Nat Med. 23:723.
  2. Muczynski V, et al. 2017. Blood. 129:2443.
  3. Iftakhar-E-Khuda I, et al. 2016. Proc Natl Acad Sci U S A. 113:10643.
  4. Nellimarla S, et al. 2015. J Immunol. 195:3858.
  5. Bonilla DL, et al. 2013. Immunity. 39:537.
Gene ID
20288 View all products for this Gene ID
View information about CD204 on

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Go To Top Version: 1    Revision Date: 10/15/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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