- ZAQ5 (See other available formats)
- Other Names
- CMRF35-like molecule 9, CLM-9, CD300g, D11Ertd736e
- Rat IgG2a, κ
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- Product Citations
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CD300LG, also known as nepmucin (a not expressed in Peyer's patches mucin), is a member of the CD300 antigen like family. It is a 50-95 kD glycoprotein with one IgV like domain and one mucin like domain and four isoforms are produced by alternative splicing. Nepmucin is expressed on high endothelial venules (HEV) from lymph nodes, but is absent on HEV from the Peyer's patches. CD300LG mediates lymphocyte adhesion through the intraction of its Ig domain with ICAM-1 and rolling on HEVs through the interaction of its mucin like domain with L-selectine. Also, it may play a role in the molecular transcytosis across the capillary endothelium.Product Details
- Antibody Type
- Host Species
- Nepmucin-Fc fusion protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
- The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer under optimal conditions. The solution is free of unconjugated DNA and unconjugated antibody.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
PG - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. For Proteogenomics TotalSeq™-A analysis, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
To maximize performance, centrifuge the antibody dilution (1.0 µg of antibody in 100 µl of staining buffer for every 1 million cells) before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
- Application Notes
Additional reported applications for the relevant formats include: immunoelectron microscopy of peripheral LN HEVs1.
- Additional Product Notes
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The TotalSeq™-A barcode sequence associated with clone ZAQ5 is CGGTCCGTATCATTT.
The flanking sequences are CCTTGGCACCCGAGAATTCCA, and the poly A tail, BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A, where B represents either C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
The full oligomer sequence for this product, with the specific barcode in brackets is CCTTGGCACCCGAGAATTCCA [CGGTCCGTATCATTT]BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A.
(PubMed link indicates BioLegend citation)
- Umemoto E, et al. 2006. J. Exp. Med. 203:1603. (EM)
- Jin S, et al. 2008. FEBS Lett. 582:3018.
- Umemoto E, et al. 2011. Crit. Rev. Immunol. 31:147.
- Umemoto E, et al. 2013. PLoS One 8:e83681.
AB_2783116 (BioLegend Cat. No. 147105)
- Sialomucin, member of the CD300 antigen like family, 50-95 kD. 1 IgV like domain, 1 mucin like domain. Four isoforms produced by alternative splicing.
Expressed on high endothelial venules (HEVs) from lymph nodes but absent on HEVs from Peyer's patches, some myeloid cell lines.
- Mediator of lymphocyte adhesion and rolling on HEVs, possible role in transcytosis across the capillary endothelium.
- Ligand Receptor
- Cell Type
- Endothelial cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Takatsu H, et al. 2006. Biochem. Biophys. Res. Commun. 348:183.
2. Umemoto E, et al. 2006. J. Exp. Med. 203:1603.
3. Umemoto E, et al. 2011. Crit. Rev. Immunol. 31:147.
- Gene ID
- 52685 View all products for this Gene ID
- View information about CD300LG on UniProt.org
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