TotalSeq™-A0403 anti-human CD207 (Langerin) Antibody

Pricing & Availability
Clone
10E2 (See other available formats)
Isotype
Mouse IgG1, κ
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352207 10 µg £218
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Description

CD207, also known as Langerin, is a 40 kD type II transmemberane cell glycoprotein which belongs to C-type lectin with mannose binding specificity. It is predominantly expressed on Langerhans cells and induces the formation of Birbeck granules, the Langerhans cell hallmark organelle. It is also found on several other subtypes of dendritic cells, such as dermal CD103-positive dendritic cells and splenic CD8-positive dendritic cells. Langerin is generally thought to be involved in antigen processing. Recently, it has been found that HIV captured by Langerin was internalized into Birbeck granule and degraded, which results in inhibition of HIV-1 infection and subsequent transmission.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Primary human Langerhans cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation
The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer under optimal conditions. The solution is free of unconjugated DNA and unconjugated antibody.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. For Proteogenomics TotalSeq™-A analysis, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

To maximize performance, centrifuge the antibody dilution (1.0 µg of antibody in 100 µl of staining buffer for every 1 million cells) before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension.


Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes

Additional reported application (for the relevant formats) includes: blocking the binding of HIV-1 to Langerhans cells1.

Additional Product Notes

TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The TotalSeq™-A barcode sequence associated with clone 10E2 is CATTCTTCACGGGAT.

The flanking sequences are CCTTGGCACCCGAGAATTCCA, and the poly A tail, BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A, where B represents either C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

The full oligomer sequence for this product, with the specific barcode in brackets is CCTTGGCACCCGAGAATTCCA [CATTCTTCACGGGAT]BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A.

Application References

(PubMed link indicates BioLegend citation)
  1. Witte LD, et al. 2007. Nat. Med. 13:367. (Block)
RRID
AB_2783248 (BioLegend Cat. No. 352207)

Antigen Details

Structure
40 kD glycoprotein
Distribution

Langerhans cells, dermal CD103-positive dendritic cells, and splenic CD8-positive dendritic cells

Function
Binding and processing antigen; capturing, internalizing and degrading HIV
Ligand Receptor
Antigen containing mannose, N-acetyl glucosamine, and fucose
Cell Type
Dendritic cells, Langerhans cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Valladeau J, et al. 2000. Immunity 12:71.
2. Mc Dermott R, et al. 2002. Mol. Biol. Cell. 13:317.
3. Mizumoto N, et al. 2004. J. Clin. Invest. 113:701.
4. Witte LD, et al. 2007. Nat. Med. 13:367.

Gene ID
50489 View all products for this Gene ID
UniProt
View information about CD207 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/15/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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