- 10E2 (See other available formats)
- Mouse IgG1, κ
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CD207, also known as Langerin, is a 40 kD type II transmemberane cell glycoprotein which belongs to C-type lectin with mannose binding specificity. It is predominantly expressed on Langerhans cells and induces the formation of Birbeck granules, the Langerhans cell hallmark organelle. It is also found on several other subtypes of dendritic cells, such as dermal CD103-positive dendritic cells and splenic CD8-positive dendritic cells. Langerin is generally thought to be involved in antigen processing. Recently, it has been found that HIV captured by Langerin was internalized into Birbeck granule and degraded, which results in inhibition of HIV-1 infection and subsequent transmission.Product Details
- Human, Cross-Reactivity: Cynomolgus
- Antibody Type
- Host Species
- Primary human Langerhans cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
- The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer under optimal conditions. The solution is free of unconjugated DNA and unconjugated antibody.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
PG - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. For Proteogenomics TotalSeq™-A analysis, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
To maximize performance, centrifuge the antibody dilution (1.0 µg of antibody in 100 µl of staining buffer for every 1 million cells) before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
- Application Notes
Additional reported application (for the relevant formats) includes: blocking the binding of HIV-1 to Langerhans cells1.
- Additional Product Notes
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The TotalSeq™-A barcode sequence associated with clone 10E2 is CATTCTTCACGGGAT.
The flanking sequences are CCTTGGCACCCGAGAATTCCA, and the poly A tail, BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A, where B represents either C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
The full oligomer sequence for this product, with the specific barcode in brackets is CCTTGGCACCCGAGAATTCCA [CATTCTTCACGGGAT]BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A.
(PubMed link indicates BioLegend citation)
- Witte LD, et al. 2007. Nat. Med. 13:367. (Block)
AB_2783248 (BioLegend Cat. No. 352207)
- 40 kD glycoprotein
Langerhans cells, dermal CD103-positive dendritic cells, and splenic CD8-positive dendritic cells
- Binding and processing antigen; capturing, internalizing and degrading HIV
- Ligand Receptor
- Antigen containing mannose, N-acetyl glucosamine, and fucose
- Cell Type
- Dendritic cells, Langerhans cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
1. Valladeau J, et al. 2000. Immunity 12:71.
2. Mc Dermott R, et al. 2002. Mol. Biol. Cell. 13:317.
3. Mizumoto N, et al. 2004. J. Clin. Invest. 113:701.
4. Witte LD, et al. 2007. Nat. Med. 13:367.
- Gene ID
- 50489 View all products for this Gene ID
- View information about CD207 on UniProt.org
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