TotalSeq™-A0369 anti-human CD29 Antibody

Pricing & Availability
Clone
TS2/16 (See other available formats)
Workshop
V A-S202
Other Names
Integrin β1 chain, VLA-β chain, gpIIa, ITGB1
Isotype
Mouse IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
303027 10 µg £218
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Description

CD29 is a 130 kD single chain type I glycoprotein also known as integrin β1, VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins, which include CD29, bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cattle (Bovine, Cow), Cynomolgus, Dog (Canine), (Horse reactivity), Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation
The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer under optimal conditions. The solution is free of unconjugated DNA and unconjugated antibody.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. For Proteogenomics TotalSeq™-A analysis, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

To maximize performance, centrifuge the antibody dilution (1.0 µg of antibody in 100 µl of staining buffer for every 1 million cells) before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension.


Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen tissue sections3,5, and activation of integrin β14,7,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 303010). Clone TS2/16 recognizes epitope A2.10

Additional Product Notes

TotalSeq™ reagents are designed to profile protein expression at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The TotalSeq™-A barcode sequence associated with clone TS2/16 is GTATTCCCTCAGTCA.

The flanking sequences are CCTTGGCACCCGAGAATTCCA, and the capture sequence, BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A, B represents either C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

The full oligomer sequence for this product, with the specific barcode in brackets is CCTTGGCACCCGAGAATTCCA [GTATTCCCTCAGTCA]BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208.
  3. Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP)
  4. Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ)
  5. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC)
  6. Murga M, et al. 2005. Blood 105:1992. (FC) PubMed
  7. Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ)
  8. Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ)
  9. Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity)
  10. Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130.
  11. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
  12. Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)
RRID
AB_2783160 (BioLegend Cat. No. 303027)

Antigen Details

Structure
Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α16), also associates with CD51 (αV), and α7- α9, 130 kD
Distribution

Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells

Function
Cell-cell and cell-matrix interactions
Ligand Receptor
VCAM-1, MAdCAM-1, ECM
Cell Type
Endothelial cells, Fibroblasts, Granulocytes, Lymphocytes, Mast cells, Monocytes, Platelets, Mesenchymal Stem Cells, Embryonic Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hemler M. 1990. Annu. Rev. Immunol. 8:365.
2. Hynes R. 1992. Cell 69:11.

Gene ID
3688 View all products for this Gene ID
UniProt
View information about CD29 on UniProt.org

Related FAQs

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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