- HIP8 (See other available formats)
- IV P38
- Other Names
- gpIIb, CD41a
- Mouse IgG1, κ
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- Product Citations
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CD41 is a 125/25 kD α subunit of the gpIIb/IIIa (CD41/CD61) complex. CD41 is a heterodimer composed of a heavy chain (gpIIbα) and light chain (gpIIbβ) linked by a single disulfide bond. It is a member of the integrin family primarily expressed on platelets and megakaryocytes. CD41 has been reported to be involved with platelet aggregation and platelet attachment to the ECM. CD41/CD61 complex acts as the receptor for fibrinogen, fibronectin, Von Willebrand factor, and thrombin.Product Details
- Human, African Green, Baboon, Capuchin Monkey, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
- The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer under optimal conditions. The solution is free of unconjugated DNA and unconjugated antibody.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
PG - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. For Proteogenomics TotalSeq™-A analysis, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
To maximize performance, centrifuge the antibody dilution (1.0 µg of antibody in 100 µl of staining buffer for every 1 million cells) before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and blocking of platelet aggregation2. The HIP8 antibody has been reported to block the activation of platelets by various stimuli, including collagen, and ADP.
- Additional Product Notes
TotalSeq™ reagents are designed to profile protein expression at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The TotalSeq™-A barcode sequence associated with clone HIP8 is ACGTTGTGGCCTTGT.
The flanking sequences are CCTTGGCACCCGAGAATTCCA, and the capture sequence, BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A, B represents either C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
The full oligomer sequence for this product, with the specific barcode in brackets is CCTTGGCACCCGAGAATTCCA [ACGTTGTGGCCTTGT]BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A.
(PubMed link indicates BioLegend citation)
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- McCarty OJT, et al. 2000. Blood 96:1789.
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Zhi L et al. 2013. PLoS One. 8:e79869. (IHC)
AB_2783162 (BioLegend Cat. No. 303737)
- Integrin family, α subunit of CD41/CD61 (GPIIb-IIIa) complex, 125/22 kD
- Platelet aggregation, platelet attachment to extracellular matrix
- Ligand Receptor
- Fibrinogen, fibronectin, von Willebrand factor, thrombin
- Cell Type
- Megakaryocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Denzin L, et al. 1996. J. Exp. Med. 184:2153.
2. Denzin L, et al. 1995. Cell 82:155.
3. Riberdy J, et al. 1994. J. Cell Biol. 125:1225.
- Gene ID
- 3674 View all products for this Gene ID
- View information about CD41 on UniProt.org
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