Stop Solution for TMB Substrate

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423001 60 ml (30 ml x 2 bottles) £25
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Biolegend’s Stop Solution for TMB Substrate is specifically formulated to terminate the HRP/TMB reaction and stabilizing the color development for ELISA applications. TMB is a chromogenic substrate used in ELISA. TMB can act as a proton donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as HRP. The resulting diimine causes the solution to take on a blue color, and this color change can be read at 650 nm. The reaction can be stopped by addition of stop reagent, and the color turns from blue to yellow with 2-3 fold increased absorbance values at 450 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

This solution is ready to use and requires no dilution.
Storage & Handling
Store at room temperature.


Application Notes

Each lot of Stop Solution for TMB (3,3'5,5'-tetramethylbenzidine) Substrate has been tested by ELISA. The TMB stop solution will stop TMB and HRP (horseradish peroxidase) reaction by adding the same volume as the TMB substrate added to each well. Addition of stop solution changes the color from blue to yellow. Read absorbance at 450 nm within 60 minutes. If the reader can read at 570 nm, the absorbance at 570 nm can be subtracted from the absorbance at 450 nm.

Additional Product Notes

Caution: Caustic material, strong acid. Wear eye, hand, and face protection.

Application References

(PubMed link indicates BioLegend citation)
  1. Kiianitsa K, et al. 2014. Nucleic Acids Res. 42:108. PubMed

Product Citations
  1. Sinani G, et al. 2017. Int J Pharm. 10.1016/j.ijpharm.2017.06.053. PubMed
  2. Maizels K 2014. Nucleic Acids Res. 42:108. PubMed

Antigen Details

Gene ID

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Go To Top Version: 1    Revision Date: 04/03/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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