Recombinant Rat IL-7 (carrier-free)

Pricing & Availability
Other Names
IL7, Interleukine 7, Interleukin-7, Lymphopoietin-1, PBGF
Ave. Rating
Submit a Review
Product Citations
publications
Rat_IL-7_RECOM_CF_1_110718_updated
Recombinant rat IL-7 induces the proliferation of PHA activated human peripheral blood lymphocytes.
  • Rat_IL-7_RECOM_CF_1_110718_updated
    Recombinant rat IL-7 induces the proliferation of PHA activated human peripheral blood lymphocytes.
  • Rat_IL-7_RECOM_CF_2_110718_updated
    Stability testing for recombinant rat IL-7. Recombinant rat IL-7 was aliquoted in DPBS at 0.2 mg/mL and one aliquot was kept at 4°C (control) and another was frozen and thawed four times (4X Freeze/Thaws). After this procedure, the samples were tested by the induction of proliferation of PHA activated human peripheral blood lymphocytes.
Cat # Size Price Quantity Avail. Save
777702 10 µg £118
Check Availability


Need larger quantities of this item?
Request Bulk Quote
777704 25 µg £218
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

IL-7 was initially described as a stromal derived factor which is capable of inducing the growth of pre-B cells in vitro. IL-7 acts on a variety of cells through its receptor (IL-7R), a heterodimer consisting of IL-7Rα (CD127) and a common γc chain (CD132) shared by other cytokine (IL-2, IL-4, IL-9, IL-15, and IL-21) receptors. In addition, IL-7Rα is shared with TSLP. The generation of IL-7-deficient and IL-7Rα-deficient mice and monoclonal antibody blocking experiments confirmed the requirement of IL-7 for B-cell development in mice. Mutations in IL-7Rα observed in patients with severe combined immunodeficiency (SCID) also underlies the importance of IL-7 signaling in T-cell development in humans. However, the presence of B cells in these individuals suggests important differences between the role of IL-7 in murine and human lymphocyte development. Thus, although human B-cell development does not appear to require IL-7, immature human B cells do proliferate in response to IL-7. Nevertheless, most recent information suggests that IL-7 dependence in human lymphopoiesis increases during development in cord blood and bone marrow. The amino acid sequence of rat IL-7 possesses approximately 89 % and 70 % identity with mouse and human, respectively. Microarray data identified IL-7 transcripts in preovulatory follicles in rats, while IL-7Rα (CD127) and γc chain (CD132) were detected in granulosa cells and preovulatory oocytes. In vitro studies showed that IL-7 induces oocyte maturation and inhibits apoptosis in granulosa cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Source
Rat IL-7, amino acids Asp26 – Ile154 (Accession # P56478) was expressed in CHO cells. The amino-terminal contains 8His-2(GGGS) IEGR tag.
Molecular Mass
The 149 amino acid recombinant protein has a predicted molecular mass of approximately 16.9 kD. The DTT-reduced and non reduced protein migrates at approximately at 20 – 25 and 17 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is His.
Purity
>95 %, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in DPBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
Recombinant rat IL-7 induces the proliferation of PHA activated human peripheral blood lymphocytes. The ED50 = 0.3 – 1.5 ng/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Cytokine
Distribution

IL-7 is produced by epithelial cells in thymus, bone marrow, and intestine. Additional sites of IL-7 production include epithelial goblet cells, keratinocytes, fetal liver, adult liver, dendritic cells, skeletal muscle cells, fibroblastic reticular cells, and follicular dendritic cells.

Function
IL-7 induces proliferation of human immature B cells, and it is critical for T-cell development and peripheral T-cell homeostasis.
Interaction
Immature B cells, T cells, naïve and memory CD4 and CD8 cells, dendritic cells.
Ligand Receptor
IL-7Rα (CD127), common gamma chain (γc or CD132).
Bioactivity
Rat IL-7 induces the proliferation of PHA activated human peripheral blood lymphocytes.
Biology Area
Cell Biology, Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References
  1. Park LS, et al. 1990. J Exp Med. 171:1073.
  2. Fry TJ and Mackall CL, 2002. Blood. 99:3892.
  3. Lai L, et al. 2006. Blood. 107:1776.
  4. Link A, et al. 2007. Nat Immunol. 8:1255.
  5. Wofford J, et al. 2008. Blood 111:2101.
  6. Parrish YK, et al. 2009. J Immunol. 182:4255.
  7. Saini M, et al. 2009. Blood. 113:5793.
  8. Guimond M, et al. 2009. Nat Immunol. 10:149.
  9. Cheng Y, et al. 2011. Biol Reprod. 84:707.
Gene ID
25647 View all products for this Gene ID
UniProt
View information about IL-7 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 11/07/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login / Register
Forgot your password? Reset password?
Create an Account