Recombinant Mouse XCL1 (carrier-free)

Pricing & Availability
Other Names
XCL1, Lymphotactin, Ltn, ATAC, SCYC1, SCM-1, SCM-1a, lymphotaxin, C motif chemokine 1, Small-inducible cytokine C1
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Product Citations
publications
Mouse_XCL1_CF_RECOM_061419
The ED50 for mouse XCL1 is 0.2 - 1.2 ng/mL, as measured by its ability to chemoattract Ba/F3 mouse pro B cells transfected with human XCR1.
  • Mouse_XCL1_CF_RECOM_061419
    The ED50 for mouse XCL1 is 0.2 - 1.2 ng/mL, as measured by its ability to chemoattract Ba/F3 mouse pro B cells transfected with human XCR1.
  • Mouse_XCL1_CF_RECOM_2_061419
    Stability Testing for Recombinant Mouse XCL1. Recombinant Mouse XCL1 was aliquoted in PBS, pH7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaws) and compared to the control that was kept at 4°C (control). The samples were tested for their ability to chemoattract Ba/F3 mouse pro B cells transfected with human XCR1. The ED50 for this effect is 0.2 - 1.2 ng/mL.
Cat # Size Price Quantity Avail. Save
783502 10 µg £84
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783504 25 µg £165
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Description

XCL1, also known as lymphotactin, is an inflammatory chemokine secreted by T cells and natural killer (NK) cells. Early reports described XCL1 as a mediator of T cell and NK cell migration, but recent studies suggest a highly specialized role for XCL1 and its receptor XCR1. The XCL1-XCR1 axis has been shown to play an important role in the dendritic cell mediated cytotoxic immune response and in the formation of self-tolerance mechanisms through the development of regulatory T cells within the thymus. XCL1 also acts as a conformation-dependent inhibitor of HIV-1. Unlike other chemokines, XCL1 conformation interchanges between conserved chemokine folds and an unrelated dimeric structure under physiological conditions. The XCL1 monomer binds XCR1 with a high affinity, whereas the dimer form binds glycosaminoglycans (GAGs) with a high affinity, but fails to activate XCR1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Source
Mouse XCL1, amino acid Val22-Gly114 (accession #AAA56752.1), was expressed in E. coli.
Molecular Mass
The 93 amino acid recombinant protein has a predicted molecular mass of approximately 10.1 kD. The DTT-reduced and non-reduced protein migrates at approximately 12 kD and 15 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Val.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 μm filtered protein solution is in PBS pH 7.2.
Endotoxin Level
Less than 0.10 EU per μg cytokine as determined by the LAL method.
Concentration
10-25 µg sizes are bottled at 200 µg/mL.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
The ED50 is 0.2 -1.2 ng/mL, as measured by its ability to chemoattract Ba/F3 mouse pro B cells transfected with human XCR1.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution

Spleen, peripheral leukocytes, CD4+ and CD8+ T cells, and natural killer (NK) cells.

Function
T cell and NK cell recruitment.
Interaction
T cell, NK cell, and Dendritic cells.
Ligand/Receptor
XCR1
Bioactivity
Chemoattract Ba/F3 mouse pro B cells transfected with human XCR1.
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
16963 View all products for this Gene ID
UniProt
View information about XCL1 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 06/14/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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