Recombinant Mouse SLAM (CD150) (carrier-free)

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Other Names
Signaling lymphocytic activation molecule family member 1, Signaling lymphocytic activation molecule, SLAM, SLAM family member 1, SLAMF1, CD150
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Recombinant mouse SLAM/CD150 induces IL-4 in D10.G4.1 cells in the presence of anti-mouse CD3ε (Cat.No. 100313). The ED50 for this effect is 0.13 – 0.75 µg/mL.
  • Mouse_SLAM_CD150_CF_RECOM_1_081419.png
    Recombinant mouse SLAM/CD150 induces IL-4 in D10.G4.1 cells in the presence of anti-mouse CD3ε (Cat.No. 100313). The ED50 for this effect is 0.13 – 0.75 µg/mL.
  • Mouse_SLAM_CD150_CF_RECOM_2_081419.png
    Stability Testing for Recombinant Mouse SLAM/CD150. Recombinant mouse SLAM/CD150 was aliquoted in PBS, pH7 at 0.2 mg/mL.One aliquot was frozen and thawed four times (4x Freeze/Thaws) and compared to the control that was kept at 4°C (control). The samples were tested for their ability to induce IL-4 in D10.G4.1 cells in the presence of anti-mouse CD3ε (Cat.No. 100313). The ED50 for this effect is 0.13 – 0.75 µg/mL.
Cat # Size Price Quantity Avail. Save
783402 10 µg £54
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783404 25 µg £111
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783406 100 µg £400
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783408 500 µg £1,058
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Description

SLAM, also known as CD150, is a type I transmembrane glycoprotein, belonging to the member of the immunoglobulin superfamily and CD2 family.  SLAM is expressed on thymocytes, T cell subsets, B cells, dendritic cells, macrophages, and hematopoietic stem cells. SLAM expression has been shown to be maintained on Th1 but not Th2 clones and at a relatively high level in T regulatory cells. The expression level of SLAM is upregulated in activated T cells and LPS/IFNγ-activated macrophages. It has shown that SLAM is a self-ligand receptor and it is involved in B cell co-stimulation, proliferation, immunoglobulin production, and signal transduction.  The cytoplasmic domain of SLAM contains consensus tyrosine motifs, ITSM (immunoreceptor tyrosine-based switch motifs), which function as docking sites and interact with distinct SH2-containing molecules, including some tyrosine phosphatases, kinases, and adaptors. SLAM-associated protein (SAP/SH2D1A), as an adaptor molecule, regulates B cell differentiation. Engagement of SLAM by specific antibodies or soluble/membrane-bound forms of SLAM strongly enhances anti-CD40-induced B cell proliferation and Ig production. SLAM signaling is also important for T cell proliferation, IFN-γ production and Th1 priming. Antibodies against SLAM have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. SLAM is also required for IL-4 production by GC (germinal centers) T follicular helper cells and for TCR-induced IL-4 production by CD4 T cells. SLAM functions as a microbial sensor to control bacterial phagosomes in macrophages. Additionally, homotypic interactions of SLAM, recruiting SAP and Src kinase Fyn, are also essential for expansion and differentiation of the NKT lineage. Furthermore, SLAM interaction between invariant NKT (iNKT) cells and myeloid dendritic cells, along with antigen-driven TCR stimulation, is required for NKT2 differentiation. In combination with CD48, SLAM is a useful marker for hematopoietic stem cell studies.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Source
Mouse SLAM, amino acid (Thr25-Pro242) (Accession: #Q9QUM4.1), with a C-terminus 6His tag, was expressed in 293E cells.
Molecular Mass
The 224 amino acid recombinant protein has a predicted molecular mass of approximately 25kD. The DTT-reduced and non-reduced protein migrates at approximately 50 kD by SDS-PAGE. The predicted N-terminal amino acid is Thr.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS pH7.2.
Endotoxin Level
Less than 0.1 EU per μg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
The ED50 is 0.13 - 0.75 µg/mL, as determined by the dose-dependent co-stimulation of IL-4 production in D10.G4.1 cells in the presence of anti-mouse CD3ε (Cat. No. 100313).
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Ig superfamily, CD2 family
Distribution

T subset (activated, memory and Th1 cells), B cells, dendritic cells, macrophages, hematopoietic stem cell.

Function
B cell co-stimulation, proliferation and Ig production; T cell proliferation and cytokine production; NKT differentiation.
Interaction
T subset (activated, memory, and Th1 cells), B cells, dendritic cells, macrophages, hematopoietic stem cell; SAP, Fyn.
Ligand/Receptor
SLAM (self-ligand)
Bioactivity
Recombinant mouse SLAM/CD150 induces IL-4 in D10.G4.1 cells in the presence of anti-mouse CD3e (Cat.No. 100313).
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, NK cells, NKT cells, T cells, Th1, Th2, Tregs
Biology Area
Cell Proliferation and Viability, Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Cocks BG, et al. 1995. Nature. 376: 260.
  2. Howie D, et al. 2002. Blood. 99: 957.
  3. Morra M, et al. 2001. EMBO J. 20: 5840.
  4. Mikhalap SV, et al. 2004. Blood. 104: 4063.
  5. Punnonen J, et al. 1997. J Exp Med. 185: 993.
  6. Wang N, et al. 2004. J Exp Med. 199: 1255.
  7. Berger SB, et al. 2010. Nat Immunol. 11: 920.
  8. Griewank K, et al. 2007. Immunity. 27: 751.
  9. Baev DV, et al. 2008. J Immunol. 181: 869.
  10. Sintes J and Engel P. 2011. Immunol Cell Biol. 89: 161.
  11. Yusuf I, et al. 2010. J Immunol. 185: 190.
  12. Wang N, et al. 2004. J Exp Med. 199: 1255.
Gene ID
27218 View all products for this Gene ID
UniProt
View information about SLAM CD150 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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