Recombinant Mouse PD-L2 (B7-DC, CD273)-Fc Chimera (carrier-free)

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Other Names
CD273, Programmed cell death 1 ligand 2 (PD-L2), B7 dendritic cell molecule (B7-DC), Butyrophilin B7-DC, PDCD1 Ligand 2
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Mouse_PD-L2_CF_RECOM_100418
Recombinant mouse PD-L2 binds mouse PD-1 in a dose dependent manner. The ED50 for this effect is 0.5 - 2.5 µg/ml.
  • Mouse_PD-L2_CF_RECOM_100418
    Recombinant mouse PD-L2 binds mouse PD-1 in a dose dependent manner. The ED50 for this effect is 0.5 - 2.5 µg/ml.
  • Mouse_PD-L2_CF_RECOM_2_100418
    Stability testing for Mouse PD-L2/ CD273-Fc Chimera. Mouse PD-L2 was aliquoted in DPBS at 0.2 mg/mL; one aliquot was kept at 4°C (control) and another was frozen and thawed four times (4X Freeze/thaws). After this procedure, the samples were tested by binding assay. Mouse recombinant PD-L2 binds recombinant mouse PD-1 in a dose dependent manner.
Cat # Size Price Quantity Avail. Save
778202 10 µg £34
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778204 25 µg £57
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778206 100 µg £218
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778208 500 µg £672
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Description

PD-L2 is a type I transmembrane protein of 247 amino acids, and it is a member of the B7 family. Mouse PD-L2 has 70% amino acid identity to its human orthologue. PD-L2 is the second ligand for PD-1, and its binding to PD-1 leads to the inhibition of T cell receptor–mediated lymphocyte proliferation and cytokine secretion. PD-L2/PD-1 interaction suppresses immune responses against autoantigens and tumors and plays an important role in the maintenance of peripheral immune tolerance. Antibody blocking of PD-L2 on dendritic cells results in enhanced T cell proliferation and cytokine production. Interaction of PD-1 by PD-L1-Ig or PD-L2-Ig fusion protein has an inhibitory effect on proliferation and cytokine production during anti-CD3-mediated stimulation. PD-L2 is expressed in biopsies from human asthmatics and lungs of aeroallergen-treated mice and its expression correlates with the asthma severity. In vivo blockade of PD-L2 reduces the severity of allergen-driven airway hyperresponsiveness (AHR).  Blockage of PD-L2 induces IL-12 production by DC; IL-12 may limit the severity of allergen-induced AHR antagonizing IL-13. In addition, blockade of PD-1 does not have an effect on AHR; therefore, PD-L2 controls asthma severity in a PD-1 independent manner. PD-L2 binds to a second receptor, RGMb (repulsive guidance molecule b) which is expressed in lung interstitial macrophages and alveolar epithelial cells and is a co-receptor for bone morphogenetic proteins BMP2/4. Blockade of the RGMb/PD-L2 interaction significantly impairs the development of respiratory tolerance since the initial T cell expansion is inhibited.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
293E cells
Source
Mouse PD-L2, amino acids (Leu20-Arg219) (Accession # NP_067371) was expressed in 293E cells. The carboxyl-terminus contains a linker-IgG Fc.
Molecular Mass
The 439 amino acid recombinant protein has a predicted molecular mass of approximately 49.4 kD. The DTT-reduced and non-reduced protein migrate at approximately 70 kD and 140 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Leu.
Purity
> 95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE and HPLC analyses.
Formulation
0.22 µm filtered protein solution is in DPBS pH 6.8.
Endotoxin Level
Less than 0.10 EU per µg of protein as determined by the LAL method.
Concentration
10-25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are bottled at the concentration indicated on the vial. (Please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
The bioactivity was measured by functional ELISA. Recombinant mouse PD-L2 binds mouse PD-1 in a dose dependent manner. The ED50 = 0.5 - 2.5 µg/mL.
Application

Bioassay

Additional Product Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Dimer
Distribution

Immature dendritic cells, mature dendritic cells, subset of macrophages, placental endothelium, and medullary thymic epithelial cells.

Function
Inhibits T cell mediated proliferation and cytokine production by CD4+ T cells. Regulate T cell responses. PD-L2 expression is up-regulated on antigen-presenting cells by IFNg.
Interaction
Antigen-stimulated T and B cells, regulatory T cells, follicular T and B cells, dendritic cells, and monocytes.
Ligand Receptor
PD-1 (CD279), RGMb.
Bioactivity
Recombinant mouse PD-L2 binds PD-1.
Cell Type
Dendritic cells, Endothelial cells, Epithelial cells, Macrophages
Biology Area
Immunology
Molecular Family
CD Molecules, Soluble Receptors, Immune Checkpoint Receptors
Antigen References
  1. Latchman Y, et al. 2001. Nat. Immunol. 2:261.
  2. Brown JA, et al. 2003. J. Immunol. 170:1257.
  3. Youngnak P, et al. 2003. Biochem. Biophys. Res. Commun. 307:672.
  4. Singh AK, et al. 2011. Allergy. 66:155.
  5. Lewkowich IP, et al. 2013. Mucosal Immunol. 6:728.
  6. Xiao Y, et al. 2014. J. Exp. Med. 211:943.
  7. Bardhan K, et al. 2016. Front Immunol. 7:550.
Gene ID
58205 View all products for this Gene ID
UniProt
View information about PD-L2 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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