Recombinant Mouse N-Cadherin-Fc Chimera (carrier-free)

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Other Names
Cadherin-2, CDH2, Neuronal Cadherin, NCAD, Neuronal Calcium-dependent adhesion protein, CDHN
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Mouse_N-Cadherin_RECOM_1_102618
Immobilized Recombinant Mouse N-Cadherin supports the adhesion of U-118-MG cells in a dose dependent manner. ED50 for this effect is 0.2 - 0.8 µg/mL. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells.
  • Mouse_N-Cadherin_RECOM_1_102618
    Immobilized Recombinant Mouse N-Cadherin supports the adhesion of U-118-MG cells in a dose dependent manner. ED50 for this effect is 0.2 - 0.8 µg/mL. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells.
  • Mouse_N-Cadherin_RECOM_2_102618
    Stability testing for Mouse N-Cadherin. Mouse N-Cadherin was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaws), and compared to a control kept at 4°C (control). The samples were tested for their ability to support the adhesion of U-118-MG cells.
Cat # Size Price Quantity Avail. Save
778402 10 µg £64
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778404 25 µg £101
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778406 100 µg £346
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778408 500 µg £806
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Description

N-Cadherin (NCAD), also known as Cadherin-2 (CDH2), is a type I membrane protein that preferentially interacts in a homophilic manner. It is a member of the Cadherin super family, a family of calcium dependent cell adhesion molecules. N-Cadherin is primarily expressed in mesenchymal tissues such as neural tissue, retina, endothelial cells, fibroblasts, osteoblasts, mesothelium, myocytes, limb cartilage, oocytes, spermatids, and Sertoli cells. Human N-Cadherin is a 906 amino acid protein with a 25 amino acid signal peptide, a 134 amino acid N-terminal propeptide, a 565 amino acid extracellular domain, a 21 amino acid transmembrane domain, and a 161 amino acid cytoplasmic domain. N-Cadherin shares 98% amino acid sequence identity with both mouse and rat N-Cadherin within the extracellular domain. The N-Cadherin ectodomain can be cleaved by MMP24 which leads to the generation of a soluble 90 kD N-terminal soluble fragment and a 45 kD membrane-bound carboxy-terminal fragment 1. The Carboxy-terminal fragment is further cleaved by gamma-secretase into a 35 kD fragment. MMP24 mediated N-Cadherin cleavage in neural stem cells affects N-Cadherin mediated anchorage of neural stem cells to ependymocytes in the adult, leading to the modulation of neural stem cell quiescence. N-Cadherin is not only involved in cell-cell adhesion, but also in cell differentiation, embryogenesis, migration, invasion and signal transduction. Fibroblast growth factor receptor interaction with N-Cadherin has been implicated in contact-dependent survival of ovarian granulosa cells in a FGFR dependent manner. N-cadherin/FGFR also have a pivotal role in promoting metastasis through differential regulation of ERK and AKT. Due to N-Cadherin’s influence on cell migration and invasion, an upregulation of N-Cadherin has been shown to be common amongst aggressive cancer types. N-Cadherin promotes the survival of cancer cells by accelerating migration and invasion of cancer cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Source
Mouse N-Cadherin, amino acids Met1-Ala724 (Accession # NM_007664.4) with a C-terminal human IgG Fc (Pro100-Lys330) was expressed in 293E cells.
Molecular Mass
The recombinant protein consists of 804 amino acid mature form and 938 amino acid pro-protein with predicted molecular mass of approximately 88.6 kD and 103.9 kD respectively. The DTT-reduced proteins migrate at approximately 110 kD and 130 kD, and non-reduced proteins migrate at approximately 240-270 kD by SDS-PAGE. The predicted N-terminal amino acid is Asp and Ser.
Formulation
0.22 μm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per μg protein as determined by the LAL method.
Concentration
10-25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial. (Please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.2 - 0.8 μg/mL as measured by the ability of immobilized protein to support the adhesion of U-118-MG cells. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Homodimer
Distribution

Primarily expressed in mesenchymal tissue such as: neural tissue, retina, endothelial cells, fibroblasts, osteoblasts, mesothelium, myocytes, limb cartilage, oocytes, spermatids, and Sertoli cells.

Function
Neuronal calcium-dependent cell adhesion protein
Interaction
N-Cadherin expressing cells, FGFR
Bioactivity
Measured by its ability to support the adhesion of U-118-MG cells
Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Arregui C, et al. 2000. J. Cell Biol. 149:1263-74.
  2. García-Castro MI, et al. 2000. Science 288:1047-51.
  3. Hayashi T, Carthew RW. 2004. Nature 431:647-52.
  4. Derycke LD, Bracke ME. 2004. Int. J. Dev. Biol. 48:463-76.
  5. Porlan E, et al. 2014. Nat. Cell Biol. 16:629-38.
  6. Qian X, et al. 2014. Oncogene 33:3411-21.
  7. Nguyen T, Mège RM. 2016. Eur. J. Cell. Biol. 95:415-426.
Gene ID
12558 View all products for this Gene ID
UniProt
View information about N-Cadherin on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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