Recombinant Mouse LIF (carrier-free)

Pricing & Availability
Regulatory Status
Other Names
MLPLI (melanoma-derived LPL inhibitor), DIA (differentiation-inducing factor), LIF (leukemia inhibitory factor)
Ave. Rating
Submit a Review
Product Citations
Mouse leukemia inhibitory factor (LIF) inhibits proliferation of M-1 cells in a dose dependent manner. The ED50 of BioLegend's mouse LIF is 0.05 - 0.2 ng/mL.
  • LIF_Mouse_Recombinant_BA_071414.jpg
    Mouse leukemia inhibitory factor (LIF) inhibits proliferation of M-1 cells in a dose dependent manner. The ED50 of BioLegend's mouse LIF is 0.05 - 0.2 ng/mL.
Cat # Size Price Quantity Check Availability Save
554002 10 µg £97
Check Availability

Need larger quantities of this item?
Request Bulk Quote
554004 25 µg £153
Check Availability

Need larger quantities of this item?
Request Bulk Quote
554006 100 µg £526
Check Availability

Need larger quantities of this item?
Request Bulk Quote
554008 500 µg £1280
Check Availability

Need larger quantities of this item?
Request Bulk Quote

Leukemia inhibitory factor (LIF) is a 20 kD protein that belongs to the IL-6 receptor family. It binds to a heterodimeric receptor composed of a type I transmembrane ligand-binding subunit, the LIF-specific subunit gp190 (LIFR), and the type I transmembrane signal transducing subunit gp130, shared with the other members of the IL-6 family. It especially signals through STAT3 and JAK kinases. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissues. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal. LIF can be upregulated by proinflammatory cytokines such as TNF-α and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Product Details
Technical Data Sheet (pdf)

Product Details

Mouse LIF, amino acids (Ser24-Phe203) (Accession# NP_032527.1), was expressed in E. coli.
Molecular Mass
This 180 amino acid protein has a predicted molecular mass of approximately 20 kD. The protein migrates at approximately 20 kD in DTT-reducing conditions and approximately 18 kD in non-reducing condition by SDS-PAGE. The predicted N-terminal amino acid is Ser.
>98%, as determined by Coomassie stained SDS-PAGE.
0.22 µm filtered protein solution is in PBS, pH 7.4.
Endotoxin Level
Less than 0.1 EU per µg of protein as determined by the LAL method.
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Mouse LIF inhibits proliferation of M-1 cells. ED50 = 0.05 - 0.2 ng/mL.


Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at

Product Citations
  1. Dhakal P, et al. 2020. Proc Natl Acad Sci U S A. 23952:117. PubMed
  2. Cai W, et al. 2022. J Clin Invest. 132:. PubMed
  3. Kelleher A, et al. 2017. Proc Natl Acad Sci U S A. 114(6):E1018-E1026. PubMed
  4. Jefferson WN, et al. 2020. Environ Health Perspect. 128:37001. PubMed
  5. Wen X, et al. 2022. Front Cell Dev Biol. 10:879428. PubMed
  6. Li R, et al. 2021. iScience. 24:103487. PubMed
  7. Dhakal P, et al. 2021. Int J Mol Sci. 22:. PubMed
  8. Abe H, et al. 2019. Nat Commun. 10:2824. PubMed

Antigen Details


Expressed in the trophectoderm of the developing embryo, activated CD4+ T cells.

Inhibits embryonic stem cell differentiation, induces the terminal differentiation of myeloid leukemic cells.
Embryonic stem cells.
mLIF inhibits proliferation of M1 cells.
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Moreau JF, et al. 1988. Nature 336:690.
2. Tomida M, et al. 1993. FEBS Lett. 334:193.
3. Metcalfe SM. 2011. Genes Immun. 12:157.
4. Voyle RB, et al. 1999. Exp. Cell Res. 249:199.
5. Slaets H, et al. 2010. Trends Mol. Med. 16:493.

Gene ID
16878 View all products for this Gene ID
View information about LIF on

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 4    Revision Date: 05/17/2022

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account