Recombinant Mouse FGF-10 (carrier-free)

Pricing & Availability
Other Names
Fibroblast Growth Factor-10, FGF10, Keratinocyte growth factor 2, KGF-2, KGF2
Ave. Rating
Submit a Review
Product Citations
publications
FGF-10_CF_Mouse_RECOM_BA_033115
MCF-7 cell proliferation induced by mouse FGF-10 in the presence of 2 µg/ml heparin.
  • FGF-10_CF_Mouse_RECOM_BA_033115
    MCF-7 cell proliferation induced by mouse FGF-10 in the presence of 2 µg/ml heparin.
See competitor comparison data...
Cat # Size Price Quantity Avail. Save
751002 10 µg £72
Check Availability


Need larger quantities of this item?
Request Bulk Quote
751004 25 µg £130
Check Availability


Need larger quantities of this item?
Request Bulk Quote
751006 100 µg £400
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

FGF-10 is a member of the fibroblast growth factor family and belongs to the FGF-7 subfamily. The mitogenic activity of FGF-10 is similar to that of FGF-7. It has no mitogenic activity for NIH/3T3 cells. FGF-10 acts as a paracrine growth factor and plays multiple roles in organogenesis. FGF-10 is essential for embryonic lung branching morphogenesis. During embryonic lung development, FGF-10 is expressed in the distal mesenchyme and acts on the distal lung epithelial progenitors to prevent them from differentiating into proximal epithelial cells by inducing Sox9 and repressing Sox2 expression. FGF-10 knockout mice die at birth due to its lack of lung and limb formation. FGF-10 takes an important role in adult lung repair and its regulation is critical for the amplification of epithelial progenitors. FGF-10 also controls adipocyte differentiation by inhibiting C/EBPβ. The development of embryonic white adipocyte tissue of FGF-10 knockout mice is greatly impaired. Additionally, FGF-10 participates in the development of pancreas, stomach, skin, hair follicle, and wound healing as well. The increased expression of FGF-10 and the receptor FGFR2-IIIb are associated with several diseases such as prostate, breast, pancreas, and colorectal cancer, which suggests a role of FGF-10 in carcinogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse FGF-10, amino acids (Ser62-Thr209) (Accession# BC048229) with an N-terminus Met, was expressed in E. coli.
Molecular Mass
The 149 amino acid recombinant protein has a predicted molecular mass of approximately 17.1 kD. The protein migrates approximately at 17 kD in DTT-reducing and non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Met.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
20 mM MOPS in pH 7.2, 150 mM Na2SO4, 0.2 mM EDTA, 2 mM DTT, and in 10% glycerol.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
The ED50 = 0.2 - 1.0 ng/ml, corresponding to a specific activity of 1.0 - 5.0 x 106 units/mg, as determined by a dose-dependent stimulation of MCF-7 cell proliferation in the presence of 2 µg/ml heparin.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Growth factor.
Distribution

FGF-10 is expressed and secreted by the mesenchyme fibroblasts during embryonic development. It is secreted by 3T3L1 preadipocytes.

Function
FGF-10 is an epithelial-mesenchymal signaling molecule and plays imporant roles in multi-organ development. Its expression is regulated by Wnt signaling, Pax9, and c0Myc.
Interaction
FGF-10 targets urothelial cells, epithelial cells, epidermal cells, keratinocyte, and smooth muscle scells. FGF-10 interacts with FGFBP1.
Ligand/Receptor
FGFR2-IIIb.
Cell Type
Neural Stem Cells, Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area
Cell Biology, Neuroscience, Synaptic Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Sekine K, et al. 1999. Nat. Genet. 21:138.
2. Petiot A, et al. 2003. Development 130:5493.
3. Tagashira S, et al. 1997. Gene 197:390.
4. El Agha E adn Bullusci S. 2014. Scientifica 2014:53879.
5. Ramasamy SK, et al. 2007. Dev. Biol. 307:237.
6. Yamasaki M, et al. 1999. Biochem Biphys Res Commun 258:109.
7. Emoto H, et al. 1997. J. Biol. Chem. 272:23191.

Gene ID
14165 View all products for this Gene ID
UniProt
View information about FGF-10 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 2    Revision Date: 06/24/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account