Recombinant Mouse FcγRIII (CD16) (carrier-free)

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Regulatory Status
RUO
Other Names
Fc-Gamma Receptor III-2 (CD16), Immunoglobulin G Fc Receptor III, Fc Fragment Of IgG, Low Affinity III, Receptor For, Low Affinity Immunoglobulin Gamma Receptor III-A Fc Fragment, Fc Fragment Of IgG Receptor IIIa, Fc-Gamma RIII-Alpha
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Mouse_FcgammaRIII-CD16_CF_RECOM_1_040620.png
When Ultra-LEAF™ purified mouse IgG2a, κ isotype ctrl antibody (Cat. No. 401507) is immobilized at 2.0 µg/mL, recombinant mouse FcγRIII binds in a dose-dependent manner. The EC50 range for this effect is 0.25 - 1.0 µg/mL. HRP anti-His Tag Antibody (Cat. No. 652503) was used to detect the binding.
  • Mouse_FcgammaRIII-CD16_CF_RECOM_1_040620.png
    When Ultra-LEAF™ purified mouse IgG2a, κ isotype ctrl antibody (Cat. No. 401507) is immobilized at 2.0 µg/mL, recombinant mouse FcγRIII binds in a dose-dependent manner. The EC50 range for this effect is 0.25 - 1.0 µg/mL. HRP anti-His Tag Antibody (Cat. No. 652503) was used to detect the binding.
  • Mouse_FcgammaRIII-CD16_CF_RECOM_2_040620.png
    Stability Testing for Mouse FcγRIII. Recombinant mosue FcγRIII was aliquoted in PBS at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with mosue IgG2a.
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790102 10 µg £55.00
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790104 25 µg £113.00
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Description

FcγRIII, also known as CD16, is a low affinity IgG Fc receptor for a broad range of different isotypes. It primarily exists as two distinct forms: FcγRIIIA/CD16a and FcγRIIIB/CD16b, which are encoded by two nearly identical genes in humans. The expression of the two isoforms is tissue-specific. FcγRIIIA is a 50-65 kD type I transmembrane protein that is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. FcγRIIIB is a 48 kD GPI-anchored protein. Its extracellular domain is over 95% homologous to that of FcγRIIIA, and it is expressed specifically on neutrophils.  FcγRIIIA can bind aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC). Several studies indicated that the post-translational modifications of FcγRIIIA or IgG regulate FcγRIIIA-mediated functions. The glycosylation of FcγRIIIA is mandatory for high affinity binding to Fc. Additionally, lack of fucosylation of Fc glycans dramatically enhance ADCC by improving the binding of FcγRIIIA and IgG. It has been reported that the polymorphism (F176V) showed a greater binding capacity to IgG, leading to increasing antibody-dependent NK cell cytotoxicity and susceptibility to autoimmune diseases.  The missense mutation (L66H) causes immunodeficiency 20 disease (IMD20). FcγRIIIA associates with γ and zeta chains of T-cell antigen receptor complex (CD3:TCR) and FcεRIγ on human NK cells, suggesting involvement of distinct signal-transducing function.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse FcγRIII, amino acid Ala31-Thr215 (Accession # P08508.1), with a C-terminal 10x His, was expressed in CHO cells.
Molecular Mass
The 195 amino acid recombinant protein has a predicted molecular mass of approximately 22.5 kD. The DTT-reduced protein and the non-reduced proteins migrate at approximately 45 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
Purity
>95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is in PBS, pH7.2
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
When Ultra-LEAF™ purified mouse IgG2a, κ isotype ctrl antibody (Cat. No. 401507) is immobilized at 2.0 µg/mL, recombinant mouse FcγRIII binds in a dose-dependent manner. The EC50 range for this effect is 0.25 - 1.0 µg/mL. HRP anti-His Tag antibody (Cat. No. 652503) was used to detect the binding.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Immunoglobulin family
Distribution

NK cells, activated monocytes, macrophages, neutrophils, subpopulation of T cells, immature thymocytes

Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
Interaction
FcγR subunit
Ligand/Receptor
Aggregated IgG, IgG-antigen complex
Bioactivity
Measured by its ability to bind mouse IgG2a
Antigen References
  1. Bruhns P. 2012. Blood. 119 5640.
  2. Hirano M, et al. 2007. Nat Immunol. 8:762.
  3. Takai T. 2002. Nat Rev Immunol. 2:580.
  4. Jakus Z, et al. 2008. J Immunol. 180:618.
  5. Nimmerjahn F and Ravetch JV. 2005. Science. 310:1510.
  6. Hazenbos WL, et al. 1996. Immunity. 5:181-8.
  7. Hazenbos WL, et al. 1998. J Immunol. 161:3026.
  8. Watanabe N, et al. 1999. Blood. 94:3855.
Gene ID
14131 View all products for this Gene ID
UniProt
View information about FcgammaRIII on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 1    Revision Date: 04/06/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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