- Other Names
- Coxsackievirus and Adenovirus Receptor, CAR, CVB3 binding protein, Coxsackievirus B Receptor
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- Product Citations
CXADR (coxsackie and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (IGSF). Its 218 amino acid extracellular domain consists of two immunoglobulin-like domains, V-type (D1) and C-type (D2) that share 90% amino acid sequence identity with mouse and rat CXADR. CXADR functions as a receptor for group B coxsackieviruses and subgroup C adenoviruses. It also functions as a homophilic cell adhesion molecule within junctional complexes between epithelial cells lining body cavities. CXADR is essential for tight junction integrity and normal cardiac development in mice. It is also involved in transepithelial migration of leukocytes through interaction with JAML. CXADR/JAML interaction activates gamma-delta T-cells through PI3-kinase and MAP kinases pathways. T-cell activation and subsequent proliferation leads to cytokine production stimulating epithelial tissue repair.Product Details
- Host Species
- CHO cells
- Mouse CXADR, amino acid Leu20-Gly237 (Accession # P97792), with a C-terminal human IgG1 (Pro100-Lys330) was expressed in CHO cells.
- Molecular Mass
- The 457 amino acid recombinant protein has a predicted molecular mass of approximately 51.0 kD. The DTT-reduced and non-reduced protein migrates at approximately 55 kD and 110 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Leu.
- > 95%, as determined by Coomassie stained SDS-PAGE.
- 0.22 μm filtered protein solution is in PBS, pH 7.2
- 10-25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial. (Please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for one months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
- When recombinant mouse CXADR/CAR-Fc Chimera is immobilized at 1 µg/mL, biotinylated recombinant mouse JAML binds with EC50 of 20 - 80 ng/mL in a functional ELISA.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at email@example.com.
(PubMed link indicates BioLegend citation)
- Bergelson JM, et al. 1997. Science 275:1320-3.
- Tomko RP, et al. 1997. Proc. Nat. Acad. Sci. 94:3352-6.
- Pauschinger M, et al. 1999. Circulation 99:1348-54.
- Cohen CJ, et al. 2001. Proc. Nat. Acad. Sci. 98:15191-6.
- Walters RW, et al. 2002. Cell 110:789-99.
- Zen K, et al. 2005. Mol. Biol. Cell 16:2694-703.
- Verdino P, et al. 2010. Science 329:1210-4.
- Witherden DA, et al. 2010 Science 329: 1205-10.
Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung.
- Component of the epithelial apical junction complex and functions as a homophilic cell adhesion molecule essential for the tight junction integrity. It is also involved in the transepithelial migration of leukocytes through adhesive interactions with JAML.
- Epithelial cells, leukocytes
- Ligand Receptor
- Coxsackievirus B, Adenovirus, JAML
- Measured by its ability to bind biotinylated recombinant mouse JAML
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, Soluble Receptors
- Gene ID
- 13052 View all products for this Gene ID
- View information about CXADR on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.