Recombinant Mouse CD200-Fc Chimera (carrier-free)

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Other Names
OX-2 membrane glycoprotein, OX-2, OX2, MRC OX-2, MOX1, MOX2, My033
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Mouse_CD200-Fc_CF_RECOM_1_052119
IC50 = 0.2 - 0.8 µg/mL as measured in a competition binding assay where increasing concentration of unlabeled mouse CD200 was able to competitively displace binding of biotinylated mouse CD200 (50 ng/mL) to immobilized human CD200R1.
  • Mouse_CD200-Fc_CF_RECOM_1_052119
    IC50 = 0.2 - 0.8 µg/mL as measured in a competition binding assay where increasing concentration of unlabeled mouse CD200 was able to competitively displace binding of biotinylated mouse CD200 (50 ng/mL) to immobilized human CD200R1.
  • Mouse_CD200-Fc_CF_RECOM_2_052119
    Stability testing for Mouse CD200. Recombinant mouse CD200 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (control). The samples were tested in a competition binding assay with biotinylated mouse CD200.
Cat # Size Price Quantity Avail. Save
783202 10 µg £50
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783204 25 µg £108
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783206 100 µg £296
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783208 500 µg £870
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Description

CD200 (OX-2) is a 45 kD cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF). CD200 is composed of a 202 amino acid extracellular domain containing two IgSF domains, a 27 amino acid transmembrane segment, and a 19 amino acid cytoplasmic domain.  CD200 is the only known ligand of the CD200R family and it is highly conserved in the CNS, expressed mainly by neurons and vascular endothelium. It is also present in most peripheral cell types, thymocytes, T and B cells, and dendritic cells. CD200 and CD200R interact with each other through N-terminal IgSF domains. Their interaction plays important roles in negatively regulating immune responses, and attenuating autoimmune diseases and excessive inflammatory responses against pathogens.  High expression of CD200 in the CNS is thought to be a mechanism of constitutive immune suppression, and is developmentally regulated in the mouse brain. Deficiency in neuronal CD200 may explain the chronic inflammation in human neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and multiple sclerosis. The CD200 and CD200R interaction has also been shown to play an important role in the regulation of anti-tumor immunity, and overexpression of CD200 has been reported in a number of malignancies, including CLL, as well as on cancer stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Source
Mouse CD200, amino acids Gln31-Gly232 (Accession # O54901) with a C-terminal human IgG1 Fc tag was expressed in 293E cells.
Molecular Mass
The 441 amino acid recombinant protein has a predicted molecular mass of approximately 49.4 kD. The DTT-reduced protein migrates at approximately 60 kD and and non-reduced protein migrates at approximately 120 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
IC50 = 0.2 - 0.8 µg/mL as measured in a competition binding assay where increasing concentration of unlabeled mouse CD200 was able to competitively displace binding of biotinylated mouse CD200 (50 ng/mL) to immobilized human CD200R1.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Homodimer
Distribution

Neurons and vascular endothelium in CNS, and most peripheral cell types, thymocytes, T and B cells, and dendritic cells.  

Function
Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
Interaction
Mast cells, basophils, macrophages, and dendritic cells.
Ligand/Receptor
CD200R
Bioactivity
Measured in a competition binding assay with biotinylated mouse CD200.
Cell Type
B cells, Dendritic cells, Endothelial cells, Neurons, T cells, Thymocytes
Biology Area
Cell Proliferation and Viability, Immuno-Oncology, Neuroinflammation
Molecular Family
CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Hernangomez M, et al. 2014. Curr Pharm Des. 20:4707-22.
  2. Walker DG and Lue LF. 2013. Future Neurol. 8(3) .
  3. Wright GJ, et al. 2003. J Immunol. 171: 3034-46.
  4. Vaine CA and Soberman RJ. 2014. Adv Immunol. 121:191-211.
  5. Minas K, et al. 2006. Crit Rev Immunol. 26(3): 213-30.
  6. Wang XJ, et al. 2007. J Neuroimmune Pharmacol. 2(3): 259-64.
  7. Wong KK, et al. 2010. J Leukoc Biol. 88(2): 361-72.
Gene ID
17470 View all products for this Gene ID
UniProt
View information about CD200 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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