Recombinant Mouse β-NGF (carrier-free)

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Other Names
Beta-Nerve Growth Factor, HSAN5
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598102 5 µg £54
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598104 20 µg £124
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Cat. Nos. 784002 and 784004 are available as alternatives.
Description

Neurotrophins are growth factors that modulate growth, differentiation, and survival of neurons. These structurally related proteins include neuron growth factor (NGF/β-NGF), brain derived neurogenic factor (BDNF), neurotrophin-3, and neurotrophin-4/5. They are highly homologous and share a similar structure of a tertiary folded, cysteine rich "knot". Neurotrophins are critical to the development of the central and peripheral nervous systems, and they play important roles in injury-induced neuron regeneration. β-NGF is a member of the neurotrophin family which controls growth, survival, and maturation of neurons. In peripheral and central neurons, neurotrophins are important regulators for survival, differentiation, and maintenance of nerve cells. They are detectable throughout embryogenesis and into the adult stage in the central and peripheral nervous systems. In animal models, β-NGF prevents neuronal degeneration and has become a therapeutical target in neuron regeneration studies. β-NGF is also critical for the survival and maintenance of sympathetic and sensory neurons. It is the main neurotrophin that maintains homeostasis of neurogenesis. It prevents neurons from undergoing apoptosis and promotes maturation while supporting axonal branching. Two receptors are known to interact with NGF, p75NTR and TrkA. The NGF-TrkA signaling pathway induces two pathways through activation of PI3K-Akt or Ras-MAPK. Either one of the pathways leads to CREB phosphorylation and increases expression of anti-apoptosis genes to keep neurons alive. Animal models of neurodegenerative diseases suggest that NGF can promote myelin repair of neurons in the peripheral nervous system. Clinical studies indicate that dysregulation of NGF signaling is related to Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Source
Mouse β-NGF, amino acids Ser122-Gly241 (Accession# NM_013609.2), was expressed in E. coli.
Molecular Mass
The 120 amino acids recombinant protein has a predicted molecular mass of 13.4 kD as a monomer and 26.5 kD as a dimer. The predicted N-terminal amino acid is Ser.
Purity
>98%, as determined by SDS-PAGE and HPLC analysis.
Formulation
Lyophilized
Endotoxin Level
Less than 0.1 ng per µg of protein.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
The ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1.0 x 106 units/mg, as determined by the dose-dependent stimulation of human TF-1 cells proliferation.
Application

Bioassay

Application Notes

This product is reactive with mouse and human.

Antigen Details

Structure
Dimeric growth factor.
Distribution

Cortex and hippocampus neurons, glias, B cells.

Function
β-NGF is important for maintaining neuron homeostasis.
Interaction
Neurons, astrocytes, B/T cells.
Ligand/Receptor
TrkA, p75NTR.
Bioactivity
Modulate growth, differentiation and survival of neurons.
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Selby MJ, et al. 1987. Mol. Cell. Biol. 7:3057. 
2. Adriaenssens E, et al. 2008. Cancer Res. 68:346. 
3. McDonald NQ, et al. 1993. Trends Biochem. Sci. 18:48.
4. Tuszynski MH, et al. 2004. Prog. Brain Res. 146:441.

Gene ID
18049 View all products for this Gene ID
UniProt
View information about beta-NGF on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 2    Revision Date: 04/14/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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