Recombinant Human VEGF-D (carrier-free)

Pricing & Availability
Other Names
Vascular endothelial growth factor D, c-Fos-induced growth factor, FIGF
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Product Citations
publications
VEGF-D_Human_RECOM_BA_053116
Human VEGF-D induces the proliferation of HMVEC human microvascular endothelial cells in a dose dependent manner.
  • VEGF-D_Human_RECOM_BA_053116
    Human VEGF-D induces the proliferation of HMVEC human microvascular endothelial cells in a dose dependent manner.
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760501 2 µg £50
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760502 10 µg £128
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Description

Vascular endothelial growth factor D (VEGF-D), also known as c-fos-induced growth factor (FIGF), is a member of the VEGF family. VEGF-D is structurally similar to VEGF-C and both are specific ligands for the tyrosine kinase receptor VEGFR-3 (flt-4). They are both secreted as non-covalent homodimers. The secreted VEGF-D undergoes a complex proteolytic process enabling it to also act as a ligand for VEGFR-2 (KDR/flk-1). VEGF-D is expressed in adult lungs, heart, muscles, and small intestines. It is most abundantly expressed in the fetal lungs and skin. VEGF-D exhibits mitogenic effects on vascular and lymphatic endothelial cells. VEGF-D functions in the formation of the venous and lymphatic vascular systems during embryogenesis and in the maintenance of differentiated lymphatic endothelium in adults. Both VEGF-C and VEGF-D are over-expressed in certain cancers and promote tumor lymphangiogenesis. TGF-β1 has been shown to downregulate VEGF-D expression in human lung fibroblasts.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human VEGF-D, amino acids (Phe89-Arg205) (Accession# O43915), was expressed in HEK293.
Molecular Mass
The 117 amino acid recombinant protein has a predicted molecular mass of approximately 13.1 kD. The protein migrates at approximately 20-22 kD under non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Phe.
Purity
>95%, as determined by SDS-PAGE gel and HPLC analysis.
Formulation
Lyophilized from 0.2 µm filtered protein solution in 10 mM Sodium Citrate, pH 4.0.
Endotoxin Level
Less than 1.0 EU per µg protein as determined by the LAL method.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.15 — 0.6 µg/mL as measured by the ability of the protein to induce proliferation of HMVEC.
Application

Bioassay

Antigen Details

Structure
Non-covalent homodimer.
Distribution
VEGF-D is expressed in the adult lungs, heart, muscles, and small intestines; it is most abundantly expressed in the fetal heart and lungs and skin.
Function
VEGF-D plays a key role in lymphangiogenesis, stimulates the migration, proliferation, and the survival of human endothelial cells.
Interaction
Lymphatic endothelial cells and vascular endothelial cells.
Ligand/Receptor
VEGF-R3, VEGF-R2, and Neuropilin-1.
Bioactivity
Measured by the ability of humanVEGF-D to induce the proliferation of HMVEC human microvascular endothelial cells.
Cell Type
Neural Stem Cells, Mesenchymal Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology, Angiogenesis, Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Yamada Y, et al. 1997. Genomics 42:483-488.
2. Achen MG, et al. 1998. Proc. Natl. Acad. Sci. USA 2:548-553.
3. Stacker SA, et al. 2001. Nat. Med. 2:186-91.
4. Duff SE, et al. 2003. Br. J. Cancer 89:426-430.
5. Tammela T, et al.  2005. Cardiovasc. Res. 65:550-563.
6. Roy H, et al. 2006. FEBS Letters 580:2879-87.
7. Cui Y, et al. 2014. Mol. Med. 20:120-134.

Gene ID
2277 View all products for this Gene ID
UniProt
View information about VEGF-D on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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