Recombinant Human R-Spondin 1/RSPO1 (carrier-free)

Pricing & Availability
Other Names
Roof plate-specific spondin-1, R-spondin-1, R-Spondin, RSPO1, RSPO, Cristin3, FLJ40906
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publications
Human_R-Spondin-1_CF_RECOM_0_050619
When human R-Spondin 1 is immobilized at 1.0 µg/mL, human RNF43-Fc Chimera (Cat. No. 771202) binds with an EC50 of 0.05 – 0.2 µg/mL.
  • Human_R-Spondin-1_CF_RECOM_0_050619
    When human R-Spondin 1 is immobilized at 1.0 µg/mL, human RNF43-Fc Chimera (Cat. No. 771202) binds with an EC50 of 0.05 – 0.2 µg/mL.
  • Human_R-Spondin-1_CF_RECOM_1_050619
    Stability testing for Human R-Spondin 1. Human R-Spondin 1 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with human RNF43-Fc Chimera.
  • Human_R-Spondin-1_CF_RECOM_2_050619
    Recombinant human RSPO1 (1 µg/mL) significantly amplifies recombinant mouse Wnt3a (Cat. No. 772301) induced Topflash reporter activity in HEK293T human embryonic kidney cells.
Cat # Size Price Quantity Avail. Save
783602 10 µg £77
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783604 25 µg £128
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783606 100 µg £400
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Description

R-Spondin 1 (RSPO1) was initially discovered as a spondin specifically expressed on the roof plate of the neural tube of developing embryos. The R-Spondin protein family consists of four members, R-Spondin 1 through R-Spondin 4. These proteins feature a hydrophobic signal peptide, two adjacent cysteine-rich, furin-like domains and one thrombospondin type 1 domain. These are secreted proteins that are processed through a canonical secretory pathway. R-Spondin 2 and R-Spondin 3 bind heparin sulfate proteoglycans on the cell surface whereas R-Spondin 1 and R-Spondin 4 do not show significant interaction with heparin sulfate proteoglycans. All R-Spondin family members regulate the Wnt signaling pathway probably by acting as an inhibitor of ZNRF3. R-Spondin 1 is expressed in early development at the roof plate boundary and contributes to dorsal neural tube development. R-Spondin 1 is postnatally expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in the kidney and prostate. In mice, R-Spondin 1 induces crypt cell proliferation and increases intestinal epithelial healing, providing a protective mechanism against chemotherapy induced adverse effects.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human RSPO1, amino acid Arg31-Ala263 (Accession # Q2MKA7) with a C-terminal 8x His tag, was expressed in CHO cells.
Molecular Mass
The 246 amino acid recombinant protein has a predicted molecular mass of approximately 27.1 kD. The DTT-reduced and non-reduced proteins migrate at approximately 40 kD by SDS-PAGE. The predicted N-terminal amino acid is Arg.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
When human R-Spondin 1 is immobilized at 1.0 µg/mL, human RNF43-Fc Chimera (Cat. No. 771202) binds with an EC50 of 0.05 – 0.2 µg/mL.

Human R-Spondin 1 amplifies Wnt agonist induced Topflash reporter activity in HEK293T human embryonic kidney cells.
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution

Expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen.

Function
Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors and RNF43/ZNRF3.
Ligand/Receptor
LGR4-6, RNF43/ZNRF3
Bioactivity
Measured in a binding assay with human RNF43.
Cell Type
Neural Stem Cells, Mesenchymal Stem Cells, Embryonic Stem Cells
Biology Area
Stem Cells
Molecular Family
Growth Factors
Antigen References
  1. Kim KA, et al. 2005. Science. 309: 1256-1259
  2. Parma P, et al. 2006. Nature Genet. 38: 1304- 1309
  3. Chassot A, et al. 2008. Hum Molec Genet. 17: 1264-1277
  4. Zhou WJ, et al. 2013. Nature. 501: 107-111
Gene ID
284654 View all products for this Gene ID
UniProt
View information about R-Spondin 1/RSPO1 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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