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PTX3 is part of the pentraxin family which is characterized by a cyclic multimeric structure. Penatraxins are acute phase proteins that act as pattern recognition receptors. PTX3 is a long pentraxin containing a C terminal domain similar to other members of the pentraxin family and a unique N terminal domain. Inflammatory signals stimulate PTX3 production by a variety of cells including mononuclear phagocytes, dendritic cells, fibroblasts, and endothelial cells.
Serum levels of PTX3 increase rapidly in response to a variety of inflammatory and infectious conditions including endotoxic shock and sepsis. PTX3 has been proposed as an early diagnostic marker for septic shock. PTX3 can activate the classical complement pathway, aiding in pathogen recognition by macrophages and dendritic cells. PTX3 is also believed to play an important role in protection against autoimmunity by enhancing complement-mediated clearing of apoptotic cells and restricting cross presentation. Current research has also indicated that PTX3 is involved in initiating the process of tissue repair and plays a role in female fertility. PTX3 is believed to be involved in cancer-related inflammation and high PTX3 levels have been correlated with clinical stage and severity for various cancers
- A DNA sequence encoding the human PTX3 (NP_002843.2) (Met 1-Ser 381) was fused with a polyhistidine tag at the C-terminus.
- >97% as determined by SDS-PAGE
- Lyophillized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide and protease inhibitors
- Storage & Handling
- Immediately upon receipt, store unopened vials between 2°C and 8°C and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
- Testing in progress
ELISA - Quality tested
- Recommended Usage
Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 6000 to 93.75 pg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This Human PTX3 protein is useful as a standard for a human PTX3 sandwich ELISA, using unlabeled MNB5 antibody (catalog #537902) as capture and bionylated MNB4 antibody (catalog #538004) as detection.
Dendritic cells, Endothelial cells, Fibroblasts, Monocytes
- Plays roles in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components, and female fertility.
- Cell Type
- Dendritic cells, Endothelial cells, Fibroblasts, Monocytes
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Innate Immunity
- Gene ID
- 5806 View all products for this Gene ID
- View information about PTX3 on UniProt.org