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Persephin is a disulfide-linked, homodimeric, neurotrophic factor belonging to the glial cell line-derived neurotrophic factor (GDNF) family of the TGF-β superfamily. GDNF family members also include GDNF, artemin, and neurturin. These proteins have similar functions in supporting specific neuronal populations in the central nervous system and basal forebrain with an NGF-comparable efficacy on both cholinergic and non-cholinergic neurons. They also support regulation of the maturation of the central nervous system as well as sympathetic, sensory and enteric neurons. They bind to the GDNF receptor alpha 1-4 (GFRα-1 to -4) and induce signaling through receptor tyrosine kinase RET activation. Persephin is not involved in supporting the peripheral nervous system, but it is reported to promote both the survival and growth of central dopaminergic and motor neurons, and kidney development. These effects are correlated with the expression patterns of GFRα-4 and RET. Functional GFRα-4 isoforms are found only in thyroid, adrenal medulla and portions of the central nervous system, and include GPI-linked, transmembrane and soluble forms. Binding of persephin to cell surface GFRα receptor triggers the dimerization of receptor RET and induces the autophosphorylation of the tyrosine kinase.Product Details
- Host Species
- E. coli
- Human Persephin, amino acid Ala61-Gly156 (Accession # O60542) was expressed in E. coli.
- Molecular Mass
- The 556 amino acid recombinant protein has a predicted molecular mass of approximately 60.6 kD. The DTT-reduced protein migrates at approximately 75 kD and and non-reduced protein migrates at approximately 150 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
- >95%, as determined by Coomassie stained SDS-PAGE.
- 0.22 µm filtered protein solution is in 4 mM HCl.
- Endotoxin Level
- Less than 0.1 EU per μg protein as determined by the LAL method.
- 10-25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial. (Please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
- Recombinant human Persephin induces proliferation of TT human medullary thyroid cancer cells in a dose-dependent manner with an EC50 range of 4 - 20 ng/mL.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at firstname.lastname@example.org.
Expressed in spinal cord and skeletal muscle.
- Promotes the survival of a variety of neurons including dopaminergic neurons and motor neurons.
- Cholinergic neurons, non-cholinergic neurons in basal forebrain, motor neurons.
- Measured by its ability to induce proliferation of TT human medullary thyroid cancer cells.
- Cell Type
- Neurons, Neural Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
- Biology Area
- Neuroscience, Stem Cells
- Molecular Family
- Growth Factors, Neurotrophic Factors
- Antigen References
- Jaszai J, et al. 1998. J Neurosci Res. 53:494.
- Milbrandt J, et al. 1998. Neuron. 20:245.
- Lindahl M, et al. 2001. J Biol Chem. 276:9344.
- Akerud P, et al. 2002. Mol Cell Neurosci. 21:205.
- Sariola H, et al. 2003. J Cell Sci. 116:3855.
- Golden JP, et al. 2003. Exp Neurol. 184:447.
- Wolf C, et al. 2008. J Neurochem. 107:96.
- Gene ID
- 5623 View all products for this Gene ID
- View information about Persephin on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.