- Other Names
- Neurotrophic Receptor Tyrosine Kinase 2, Neurotrophic Tyrosine Kinase, Receptor, Type 2, BDNF/NT-3 Growth Factors Receptor, BDNF-Tropomyosine Receptor Kinase B, Tropomyosin-Related Kinase B, TRKB, Trk-B, GP145-TrkB.
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- Product Citations
NTRK2 (Neurotrophic Tyrosine Kinase, Receptor, type 2), also known as TrkB (Tropomyosin related kinase B), is one of the members of the neurotrophin receptor family, which include TrkA and TrkC. All three Trk proteins exhibit a conserved subdomain that contains a signal peptide, two cysteine rich domains, a cluster of three leucine rich motifs, and two Ig-like domains in the extracellular portion. It has been shown that Trk receptor-mediated neurotrophic signaling regulates neuron physiology, cell proliferation and survival, axon and dendrite growth and patterning, and synaptic plasticity. The neurotrophins that activate NTRK2 are BDNF (Brain Derived Neurotrophic Factor), Neurotrophin-3 (NT-3), and Neurotrophin-4 (NT-4). BDNF is known to trigger NTRK2 dimerization and auto phosphorylation that leads to the activation of downstream signaling pathways. After the receptor-ligand binding, the NTRK2-BDNF complex is internalized and transported from the nerve terminal to the cell body as signaling endosomes to mediate numerous signaling events. It has been demonstrated that activation of NTRK2 can lead to down regulation of the main potassium-chloride cotransporter KCC2 in neurons, thereby increasing the intracellular Cl-concentration in lamina I neurons. Recently, it was shown that BDNF-NTRK2 signaling may play a role in the pathogenesis of post-traumatic stress disorders and mutations in the NTRK2 gene have been associated with obesity and mood disorders.Product Details
- Human NTRK2, amino acid Cys32-His430 (Accession # NM_001018064.2) was expressed in CHO cells. The C-terminus contains SR-IEGRMD-hIgG-Fc
- Molecular Mass
- The 638 amino acid recombinant protein has a predicted molecular mass of approximately 71.1 kD. The DTT-reduced and non-reduced protein migrate at approximately 90 kD and 180 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Cys.
- >96%, as determined by Coomassie stained SDS-PAGE.
- 0.22 μm filtered protein solution is in PBS pH7.2.
- Endotoxin Level
- Less than 0.1 EU per μg protein as determined by the LAL method.
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for one month, at -20&dge;C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
- Recombinant human NTRK2 binds to immobilized recombinant human BDNF in a dose-dependent manner using a functional ELISA. The ED50 for this effect is 50 – 250 ng/mL.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at firstname.lastname@example.org.
NTRK2 is expressed primarily in the nervous system. It is also found in the pancreas, skeletal muscles, and the kidney. A truncated form of TrkB (missing the tyrosine kinase domain) is found in heart, ovary, and spleen tissue. NTRK2 is also expressed in B cells of patients with B-cell chronic lymphocytic leukemia (B-CLL).
- NTRK2 interaction with its ligands (BDNF, NT-3, and NT-4) induces neuronal biological functions such as proliferation, survival, axon, and dendrite growth.
- Neurons, interacts with the neurotensin receptor 2 in B-CLL.
- BDNF, NT-3, and NT-4.
- Human NTRK2 binds to immobilized recombinant human BDNF.
- Antigen References
- Snider WD. 1994. Cell. 77:627.
- Grace PM, et al. 2014. Nat. Rev. Immunol. 14:217.
- Neveu I and Arenas E. 1996. J. Cell Biol. 133:631.
- Green CR, et al. 2013. J. Depress Anxiety. S4:006.
- Gupta VK, et al. 2013. Int. J. Mol. Sci. 14:10122.
- Pla P, et al. 2014. Front Behav. Neurosci. 8:135.
- Liu X, et al. 2014. J. Biol. Chem. 289:27571.
- Abbaci A, et al. 2018. Oncogene. 37: 756.
- Gene ID
- 4915 View all products for this Gene ID
- View information about NTRK2 on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.