Recombinant Human Notch-1-Fc Chimera (carrier-free)

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Other Names
Notch drosophila homolog of 1, Notch1, Translocation-associated notch homolog, TAN1, Neurogenic locus notch homolog protein 1
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Human_Notch-1_CF_RECOM_1_100418
When recombinant human Notch-1-Fc Chimera is immobilized at 5 µg/mL, biotinylated recombinant rat Jagged-1 binds with EC50 of 0.2 - 0.8 µg/mL in a functional ELISA.
  • Human_Notch-1_CF_RECOM_1_100418
    When recombinant human Notch-1-Fc Chimera is immobilized at 5 µg/mL, biotinylated recombinant rat Jagged-1 binds with EC50 of 0.2 - 0.8 µg/mL in a functional ELISA.
  • Human_Notch-1_CF_RECOM_2_100418
    Stability testing for recombinant human Notch-1-Fc Chimera. Recombinant human Notch-1-Fc Chimera was aliquoted in PBS, pH 7.2 at 0.2 mg/ml. One aliquot was frozen and thawed four times (4x freeze/thaws), and compared to a control kept at 4°C (control). The samples were tested in a binding assay with biotinylated recombinant rat Jagged-1.
Cat # Size Price Quantity Avail. Save
777502 10 µg £60
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777504 25 µg £101
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777506 100 µg £386
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Description

Human Notch-1 is a large type I transmembrane glycoprotein that is involved in cell fate specification, differentiation, and tissue patterning. Notch has two distinct domains, an extracellular domain (NECD) responsible for the interaction with the ligands and an intracellular domain (NICD) responsible for signal transduction. Human NECD (amino acid 19-526) shows 91% amino acid sequence identity with mouse and rat NECD. There are five Notch ligands (DLL1, DLL3, DLL4, Jagged-1 and Jagged-2) and four Notch receptors (Notch-1 to Notch-4) in mammals. Interactions of Notch receptors with membrane bound ligands result in sequential proteolysis by ADAM (disintegrin metalloprotease) and then by presenilin/gamma secretase, resulting in the shedding of NECD and the generation of soluble NICD signaling fragments. The NICD translocates to the nucleus and interacts with transcriptional factors to promote the transcription of target genes. Notch-1 is involved in angiogenesis by negatively regulating endothelial cell proliferation, migration, and angiogenic sprouting. It is also involved in the maturation of both CD4+ and CD8+ cells in the thymus. Notch-1 is important for follicular differentiation and cell fate selection within the follicle. Notch-1 functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human Notch-1, amino acid Ala19-Gln526 (Accession # P46531), with a C-terminal human IgG1 (Pro100-Lys330) was expressed in CHO cells.
Molecular Mass
The 747 amino acid recombinant protein has a predicted molecular mass of approximately 80.5 kD. The DTT-reduced and non-reduced protein migrates at approximately 90 kD and 180 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ala.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10-25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial. (Please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
When recombinant human Notch-1-Fc Chimera is immobilized at 5 µg/mL, biotinylated recombinant rat Jagged-1 binds with EC50 of 0.2 - 0.8 µg/mL in a functional ELISA.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.

Function
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination.
Ligand Receptor
Jagged-1, Jagged-2, Delta-1
Bioactivity
Measured by its ability to bind biotinylated recombinant rat Jagged-1.
Cell Type
Lymphocytes
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References
  1. Ellisen LW, et al. 1991. Cell 66:649-61.
  2. Logeat F, et al. 1998. Proc. Nat. Acad. Sci. 95:8108-12.
  3. Chan YM, Jan YN. 1998. Cell 94: 423-6.
  4. De Strooper B, et al. 1999. Nature 398: 518-22.
  5. Mumm JS, et al. 2000. Mol. Cell. 5:197-206.
  6. Das I, et al. 2004. J. Biol. Chem. 279:30771-80.
  7. Aguirre A, et al. 2010. Nature 467: 323-7.
Gene ID
4851 View all products for this Gene ID
UniProt
View information about Notch-1 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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