Recombinant Human MMP-8 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Matrix metallopeptidase 8, CLG1, MMP8, PMNL-CL, Neutrophil Collagenase, MMP
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Product Citations
publications
MMP-8_Human_RECOM_BA_1_111716
Activity of activated human MMP-8 was measured by its ability to cleave the fluorogenic peptide Mca-KPLGL(Dpa)AR.
  • MMP-8_Human_RECOM_BA_1_111716
    Activity of activated human MMP-8 was measured by its ability to cleave the fluorogenic peptide Mca-KPLGL(Dpa)AR.
  • MMP-8_Human_RECOM_BA_2_091014.jpg
    The activity of human MMP-8 was measured with 10 µM of fluorogenic MMP substrate, Mca-KPLGLDpa-AR, in the presence of 6.25, 12.5, 25, 50, 100, 200 ng of activated hMMP-8.
  • MMP-8_Human_RECOM_BA_3_091014.jpg
    The activity of human MMP-8 was measured with 10 µM of fluorogenic MMP substrate, Mca-KPLGLDpa-AR, in the presence of 6.25, 12.5, 25, 50, 100, 200 ng of activated hMMP-8.
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556102 10 µg £130.00
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556104 25 µg £257.00
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Description

MMP-8, also termed neutrophil collagenase, is a member of the matrix metalloproteinase family proteins (MMPs). Members of this family are structurally related, zinc-containing enzymes that degrade the extracellular matrix (ECM) and connective tissue proteins. MMP-8 consists of a prodomain that is cleaved upon activation, a catalytic domain containing the zinc binding site, a hinge region, and a carboxyl terminal domain with hemopexin-like repeats. Substrates of MMP-8 include type I, II, and III triple-helical collagens, gelatin peptides, proteoglycans, fibronectin, aggrecan, substance P, serpins, β-casein, and angiotensin. MMP-8 is released by neutrophils upon IL-1β IL-8, GM-CSF and TNF-α stimulation. Besides playing a role in phagocytosis, MMP-8 has been implicated in rheumatoid arthritis (RA) because of its high efficiency in infiltrating connective tissue and breaking down ECM. In fact, serum level of MMP-8 is a strong predictor of mortality in RA, as this is consistent with increased activation of neutrophils in RA. In addition, MMP-8 has been shown to play a crucial role in cardiovascular diseases by promoting atherosclerotic lesion formation. This is supported by the finding that MMP-8 knockout mice have less endothelial cells or plaque angiogenesis in the atherosclerotic plaques. In a clinical study, it was shown that the ratio of MMP-9/TIMP-1, as well as MMP-8 serum levels, could be a useful predictor of prognosis in patients with hepatocellular carcinomas.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human MMP-8, amino acids (Phe21-Gly467) (Accession# NP_002415.1), is expressed with a C-terminal His tag and a linker sequence in 293E cell lines.
Molecular Mass
This 460 amino acid recombinant protein has a predicted molecular mass of approximately 52.6 kD. The protein migrates at approximately 70 kD in DTT-reducing conditions and approximately 70 kD in non-reducing conditions by SDS-PAGE.The predicted N-terminal amino acid is Phe.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in TCN (25 mM TRIS, 10 mM CaCl2, 150 mM NaCl, pH 7.5).
Endotoxin Level
Less than 1.0 EU per µg of protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size is lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C for six months. For maximum results, quick spin vial prior to opening. Avoid repeated freeze/thaw cycles.
Activity
Activated human MMP-8 is able to cleave the peptide substrate Mca-KPLGL-Dpa-AR-NH2 with an activity above 375 pmol/min/µg.
Application

Bioassay

Application Notes

This protein is in the latent form and needs to be activated for bioassay. To activate 100 ug/mL of MMP-8 incubate the protein with 1mM of APMA at 37°C for 1h.


BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Monomer
Distribution

Neutrophils, macrophages, monocytes

Function
MMP8 degrades ECM proteins (collagen type I, II, and III, gelatin, proteoglycans, fibronectin, aggrecan, substance P, serpins, β-casein, and angiotensin); IL-1β and IL-6 stimulation activates neutrophils to release MMP-8.
Bioactivity
Human MMP-8 cleaves the peptide substrate Mca-KPLGL-Dpa-AR-NH2.
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Enzymes and Regulators
Antigen References

1. Nagase H, and Woessner JF Jr, 1999. J. Biol. Chem. 274:21491.
2. Gill SE and Parks WC, 2008. Int. J. Biochem. Cell Biol. 40:1334.
3. Mattey DL, et al. 2012. Arthritis Res. Ther. 14:R204.
4. Fang C. et al. 2013. Cardiovasc. res. 99:146.
5. Lempinen M. et al. 2013. Ann. Med. 45:482.
6. Sommer K, et al. 2013. PLos One 8:e73992.

Gene ID
4317 View all products for this Gene ID
UniProt
View information about MMP-8 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 3    Revision Date: 11/17/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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