- Other Names
- GH1, GH-1, GH, GHN, GH-N, GHB5, hGH-N, IGHD1B, Somatotropin, Growth Hormone 1
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Avail.||Save|
The human growth hormone (GH) is a pleiotropic protein that acts in different tissues such as: brain, spinal roots and peripheral nerves, heart, blood vessels, ovary, testis, intestine, kidney, muscle, bone, thymus, and liver. The human GH/CS locus is located in chromosome 17 and includes two genes for GH (GH-1 and GH2) and three chorionic somatomammotropin, hCS-1, hCS-2, hCS-5, currently called placental lactogens. GH-1 (GH-N) is expressed in pituitary and GH-2 (CG-V) in placenta. Human GH-1 is synthetized by the somatotrophs in the anterior pituitary and its release is regulated by the growth hormone releasing hormone (GHRH) and GHRH receptor (GHRHR). The GHRH is produced by the hypothalamus and the binding to the GHRH receptor releases the GH-1 stored in secretory granules. GH-1 is negatively regulated by GH release-inhibiting factor (GHRIF), and somatostatin (SST). The human growth hormone gene 1 is composed of five exons and four introns. The mature transcript encodes a biologically active form of 22 kD. Aberrant splicing in exon three produces a 20 kD active protein, and elimination of exon three results in a 17.5 kD protein that inhibits the secretion of the full active form. GH-1 promotes thymus development and engraftment of human and murine T cells in SCID mice; also induces T and B cell proliferation. GH-1 acts directly in some cells such as osteoblasts, epiphyseal growth-plate chondrocytes; some effects of GH-1 are indirectly induced by IGF-1 produced in the liver in response to GH-1. Thus, GH-1 and IGF-1 have a synergistic effect with GM-CSF in human granulopoiesis in vitro. GH-1 binds to the growth hormone receptor (GHR) and this binding induces dimerization of GHR to form a dimeric GHR:GH complex. The dimerization induces interaction with Janus2 and subsequently signals activation.Product Details
- Human Growth Hormone, amino acids (Phe27 - Phe217) (Accession # P01241 ) was expressed in 293E cells.
- Molecular Mass
- The 191 amino acid recombinant protein has a predicted molecular mass of approximately 22kD. The DTT-reduced and non-reduced protein migrate at approximately 23kD and 20kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Phe.
- > 95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE.
- 0.22 μm filtered protein solution is in PBS pH7.2.
- Endotoxin Level
- Less than 0.1 EU per μg cytokine as determined by the LAL method.
- 10-25 µg sizes are bottled at 200 µg/mL. 100 µg size is lot-specific and bottled at the concentration indicated on the vial. (Please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
- Human Growth Hormone induces the proliferation of Nb2-11 rat lymphoma cells in a dose dependent manner. The ED50 = 50 - 200 pg/mL.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at email@example.com.
Produced in the anterior pituitary by somatotrophs.
- Induces bone length, bone density, and muscle mass; regulates metabolism of lipids, carbohydrates, and body water. Induces IGF-1 from hepatocytes that mediates some GH effects.
- Hepatocytes, osteoblasts, epiphyseal growth-plate chondrocytes, T and B cells, monocytes, neutrophils. Multiple cells expressing the GH receptor.
- Ligand Receptor
- Growth Hormone Receptor (GHR)
- Induces the proliferation of Nb2 rat lymphoma cells in a dose dependent manner.
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
- Hirt H, et al. 1987. DNA. 6:59.
- de Vos AM, et al. 1992. Science. 255:306.
- Masuda N, et al. 1988. Biochim. Biophys. Acta. 949:125.
- Koo GC, et al. 2001. J. Immunol. 166:4195.
- Meazza C, et al. 2004. Pediatr. Endocrinol. Rev. 490-5.
- Solis AS, et al. 2008. J. Biol. Chem. 283:23619.
- Hattori N. 2009. Growth Horm. IGF Res. 187-97.
- Waters MJ, Brooks AJ. 2015. Biochem. J. 466:1.
- Dehkhoda F, et al. 2018. Front Endocrinol. 9:35.
- Gene ID
- 2688 View all products for this Gene ID
- View information about Growth Hormone on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.