Recombinant Human Growth Hormone (carrier-free)

Pricing & Availability
Other Names
GH1, GH-1, GH, GHN, GH-N, GHB5, hGH-N, IGHD1B, Somatotropin, Growth Hormone 1
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Product Citations
publications
Human_Growth_Hormone_RECOM_113018
Human Growth Hormone induces the proliferation of Nb2-11 rat lymphoma cells in a dose dependent manner. The ED50 = 50 - 200 pg/mL
  • Human_Growth_Hormone_RECOM_113018
    Human Growth Hormone induces the proliferation of Nb2-11 rat lymphoma cells in a dose dependent manner. The ED50 = 50 - 200 pg/mL
  • Human_Growth_Hormone_RECOM_CF_2_121918
    Stability testing for Human Growth Hormone. Recombinant Human Growth Hormone was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One was frozen and thawed four times (4x Freeze/Thaws), and compared to a control kept at 4°C (Control). The samples were tested for their ability to induce the proliferation of Nb2-11 rat lymphoma cells in a dose dependent manner. The ED50 = 20 - 100 pg/mL
Cat # Size Price Quantity Avail. Save
778002 10 µg £40
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778004 25 µg £64
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778006 100 µg £138
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Description

The human growth hormone (GH) is a pleiotropic protein that acts in different tissues such as: brain, spinal roots and peripheral nerves, heart, blood vessels, ovary, testis, intestine, kidney, muscle, bone, thymus, and liver. The human GH/CS locus is located in chromosome 17 and includes two genes for GH (GH-1 and GH2) and three chorionic somatomammotropin, hCS-1, hCS-2, hCS-5, currently called placental lactogens. GH-1 (GH-N) is expressed in pituitary and GH-2 (CG-V) in placenta. Human GH-1 is synthetized by the somatotrophs in the anterior pituitary and its release is regulated by the growth hormone releasing hormone (GHRH) and GHRH receptor (GHRHR). The GHRH is produced by the hypothalamus and the binding to the GHRH receptor releases the GH-1 stored in secretory granules. GH-1 is negatively regulated by GH release-inhibiting factor (GHRIF), and somatostatin (SST). The human growth hormone gene 1 is composed of five exons and four introns. The mature transcript encodes a biologically active form of 22 kD. Aberrant splicing in exon three produces a 20 kD active protein, and elimination of exon three results in a 17.5 kD protein that inhibits the secretion of the full active form. GH-1 promotes thymus development and engraftment of human and murine T cells in SCID mice; also induces T and B cell proliferation. GH-1 acts directly in some cells such as osteoblasts, epiphyseal growth-plate chondrocytes; some effects of GH-1 are indirectly induced by IGF-1 produced in the liver in response to GH-1. Thus, GH-1 and IGF-1 have a synergistic effect with GM-CSF in human granulopoiesis in vitro. GH-1 binds to the growth hormone receptor (GHR) and this binding induces dimerization of GHR to form a dimeric GHR:GH complex. The dimerization induces interaction with Janus2 and subsequently signals activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human Growth Hormone, amino acids (Phe27 - Phe217) (Accession # P01241 ) was expressed in 293E cells.
Molecular Mass
The 191 amino acid recombinant protein has a predicted molecular mass of approximately 22kD. The DTT-reduced and non-reduced protein migrate at approximately 23kD and 20kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Phe.
Purity
> 95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 μm filtered protein solution is in PBS pH7.2.
Endotoxin Level
Less than 0.1 EU per μg cytokine as determined by the LAL method.
Concentration
10-25 µg sizes are bottled at 200 µg/mL. 100 µg size is lot-specific and bottled at the concentration indicated on the vial. (Please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
Human Growth Hormone induces the proliferation of Nb2-11 rat lymphoma cells in a dose dependent manner. The ED50 = 50 - 200 pg/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution

Produced in the anterior pituitary by somatotrophs.

Function
Induces bone length, bone density, and muscle mass; regulates metabolism of lipids, carbohydrates, and body water. Induces IGF-1 from hepatocytes that mediates some GH effects.
Interaction
Hepatocytes, osteoblasts, epiphyseal growth-plate chondrocytes, T and B cells, monocytes, neutrophils. Multiple cells expressing the GH receptor.
Ligand Receptor
Growth Hormone Receptor (GHR)
Bioactivity
Induces the proliferation of Nb2 rat lymphoma cells in a dose dependent manner.
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References
  1. Hirt H, et al. 1987. DNA. 6:59.
  2. de Vos AM, et al. 1992. Science. 255:306.
  3. Masuda N, et al. 1988. Biochim. Biophys. Acta. 949:125.
  4. Koo GC, et al. 2001. J. Immunol. 166:4195.
  5. Meazza C, et al. 2004. Pediatr. Endocrinol. Rev. 490-5.
  6. Solis AS, et al. 2008. J. Biol. Chem. 283:23619.
  7. Hattori N. 2009. Growth Horm. IGF Res. 187-97.
  8. Waters MJ, Brooks AJ. 2015. Biochem. J. 466:1.
  9. Dehkhoda F, et al. 2018. Front Endocrinol. 9:35.
Gene ID
2688 View all products for this Gene ID
UniProt
View information about Growth Hormone on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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