Recombinant Human GDF-5/BMP-14 (carrier-free)

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Other Names
Bone morphogenetic protein 14 (BMP14), cartilage-derived morphogenetic protein 1 (CDMP1), BDA1C, Lipopolysaccharide associated protein 4 (LAP4), LPS associated protein 4, OS5, SYM1B, SYNS2, GDF5.
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Human_GDF-5_BMP-14_CF_RECOM_1_121718
Human GDF-5/BMP-14 induces alkaline phosphatase expression in the mouse chondrogenic ATDC5 cell line in a dose dependent manner. The ED50 for this effect is 0.15 - 0.9 µg/mL.
  • Human_GDF-5_BMP-14_CF_RECOM_1_121718
    Human GDF-5/BMP-14 induces alkaline phosphatase expression in the mouse chondrogenic ATDC5 cell line in a dose dependent manner. The ED50 for this effect is 0.15 - 0.9 µg/mL.
  • Human_GDF-5_BMP-14_CF_RECOM_2_121718
    Stability testing for human GDF-5/BMP-14. Recombinant human GDF-5/BMP-14 was aliquoted in 50% Acetonitrile, 0.1% TFA at 0.2 mg/mL and one aliquot was kept at 4°C (control), and another was frozen and thawed four times (4x Freeze/Thaws). After this procedure, the samples were tested for their ability to induce alkaline phosphatase expression in the mouse chondrogenic ATDC-5 cell line in a dose dependent manner. The ED50 for this effect is 0.15 - 0.9 µg/mL.
Cat # Size Price Quantity Avail. Save
779502 10 µg £47
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779504 25 µg £74
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779506 100 µg £185
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779508 500 µg £524
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Description

Growth and differentiation factor-5 (GDF-5) was initially identified in partially purified extracts from newborn calf articular cartilage. It is part of the bone morphogenetic protein (BMP) family of proteins, which are members of the TGF-β superfamily and plays a key role in skeletal development, bone formation, and stem cell differentiation. Human GDF-5 is highly homologous to its murine equivalent and the mature proteins differ only by one amino acid. GDF-5 belongs together with GDF-6 and GDF-7 to a subfamily of the BMPs. BMPs signal through heterotetrameric complex receptors, which include four type I receptors (ALK1, ALK2, ALK3, and ALK6) and three type II receptors (BMPRII, ActRIIA, and ActRIIB). GDF-5 binds specifically to BMPR1b, BMPRII, and ACTRIIA receptors forming a heterodimeric complex thus inducing the phosphorylation of SMAD1, 5, 8 and the translocation to the nucleus. GDF-5 enhances endochondral bone growth, development of limb skeletons and joints, and odontogenesis. GDF-5 stimulates osteogenic differentiation and increases VEGF levels in fat-derived stromal cells in vitro. Also, GDF-5 stimulates the proteoglycans and type II collagen production in intervertebral disc cells, and enhances cell proliferation and matrix synthesis in annulus fibrosus and nucleus pulposus cells, which inhibits degenerative disc disease. GDF-5 could be suitable for gene therapy for lumbar intervertebral disc degeneration. Mutations in the human GDF-5 gene result in a broad spectrum skeletal disorders. GDF-5 is a potent neurotrophic factor in vitro and in vivo for rat dopaminergic midbrain neurons, specifically the ones that degenerate in Parkinson's disease. Exogenous GDF-5 is neuroprotective in animal models of Parkinson's disease. Moreover, it is a key regulator of the growth and elaboration of pyramidal cell dendrites in the developing hippocampus.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Host Species
E.coli
Source
Human GDF-5/BMP14 amino acids Met-(Ala382-Arg501) (Accession # P43026) was expressed in E.coli.
Molecular Mass
The 120 amino acid recombinant protein has a predicted molecular mass of approximately 13.7 kD. The DTT-reduced and non-reduced protein migrate at approximately 14 kD and 28 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Met.
Purity
>95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 μm filtered protein solution is in 50% Acetonitrile, 0.1% TFA.
Endotoxin Level
Less than 0.1 EU per μg cytokine as determined by the LAL method.
Concentration
10-25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial. (Please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
Human GDF-5/BMP-14 induces alkaline phosphatase expression in the mouse chondrogenic ATDC5 cell line in a dose dependent manner. The ED50 for this effect is 0.15 - 0.9 µg/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Homodimer
Distribution

Predominantly expressed in cartilaginous tissues, precartilaginous mesenchymal condensation, and throughout the cartilaginous cores of the developing long bones.

Function
Skeletal development during embryogenesis, joint formation, tendon repair, and odontogenesis. MicroRNA-21 suppresses the expression of GDF-5 by inducing GDF-5 mRNA decay in chondrocytes. Noggin inhibits GDF-5.
Interaction
Chondrocytes, osteoblast, periosteum cells, fibroblasts, hippocampal pyramidal cells, and dopaminergic midbrain neurons.
Ligand Receptor
BMPR1b, BMPRII, and ACTRIIA
Bioactivity
Human GDF-5/BMP-14 induces alkaline phosphatase expression in the mouse chondrogenic ATDC5 cell line in a dose dependent manner. The ED50 for this effect is 0.15 - 0.9 µg/mL.
Cell Type
Fibroblasts, Mesenchymal cells, Neurons
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References
  1. Chang SC, et al. 1994. J. Biol. Chem. 269:28227.
  2. Hotten G, et al. 1994. Biochem. Biophys. Res. Commun. 204:646.
  3. Nishitoh H, et al. 1996. J. Biol. Chem. 35:21345.
  4. Groppe J, et al. 2002. Nature 420:636.
  5. Chen X, et al. 2006. J. Mol. Cell Cardiol. 41:26.
  6. Kleinschmidt K, et al. 2013. Biomaterials 34:5926.
  7. Osório C, et al. 2013. Development 140:4751.
  8. Zhang Y, et al. 2014. Exp. Mol. Med. 46:e79.
  9. Li YF, et al. 2015. Int. J. Clin. Exp. Pathol. 8:719.
Gene ID
8200 View all products for this Gene ID
UniProt
View information about GDF-5/BMP-14 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 12/17/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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