Recombinant Human E-Cadherin-Fc Chimera (carrier-free)

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Other Names
E-cadherin, Cadherin-1, CAD1, CDH1, CADH1, ARC-1, CD324, CAM 120/80, Epithelial cadherin, Uvomorulin, CDHE, UVO
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publications
Human_E-Cadherin-Fc_Chimera_CF_RECOM_1_012119
Immobilized Recombinant Human E-Cadherin supports the adhesion of MCF-7 cells in a dose dependent manner. ED50 for this effect is 0.35 - 1.4 µg/mL. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells.
  • Human_E-Cadherin-Fc_Chimera_CF_RECOM_1_012119
    Immobilized Recombinant Human E-Cadherin supports the adhesion of MCF-7 cells in a dose dependent manner. ED50 for this effect is 0.35 - 1.4 µg/mL. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells.
  • Human_E-Cadherin-Fc_Chimera_CF_RECOM_2_012119
    Stability testing for Human E-Cadherin. Human E-Cadherin was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested for their ability to support the adhesion of MCF-7 cells.
Cat # Size Price Quantity Avail. Save
779902 10 µg £47
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779904 25 µg £77
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779906 100 µg £218
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Description

E-cadherin, also known as cadherin-1, CDH1 and CD324, is a member of the cadherin superfamily. E-cadherin is widely expressed in epithelial cells. It is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region. E-cadherin functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. E-cadherin forms homophilic clusters via cis and trans interactions to stabilize catenin and the actin cytoskeleton in epithelial adherens junctions. E-cadherin and α-catenin can form the distinct complexes with β-catenin and plakoglobin, respectively. Mice lacking E-cadherin in the epidermis experience perinatal death and loss of barrier function. Regulating E-cadherin turnover is essential to modulate epithelial plasticity during tissue remodeling and stretching.  In bacterial pathogenesis, the ectodomain of E-cadherin mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. E-cadherin binds to the αEβ7 integrin to mediate cell adhesion.  It also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase-8, β-catenin, presenilin 1, and casein kinase II as well as other extracellular proteins including the EGF receptor. E-cadherin is phosphorylated on multiple residues (S857, S866, S870, S872), and can be proteolytically cleaved at residue D769 by caspase-3. There are several diseases associated with dysregulation of E-cadherin, including Hailey-Hailey disease and Blepharocheilodontic syndrome 1 (BCDS1).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human E-Cadherin, amino acid Asp155 - Ile707 (Accession # P12830) with a C-terminal human IgG Fc tag, was expressed in 293E cells.
Molecular Mass
The 924 amino acid recombinant protein has a predicted molecular mass of approximately 102.5 kD. The DTT-reduced proteins migrate at approximately 85 – 115 kD, and non-reduced proteins migrate at approximately 190 - 230 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH7.2
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.35 - 1.4 µg/mL as measured by the ability of immobilized protein to support the adhesion of MCF-7 cells. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Member of the cadherin superfamily
Distribution

Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas, as well as erythroid cells; plasma membrane.

Function
E-Cadherin is involved in development, bacterial pathogenesis, and tumor invasion.
Interaction
Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase-8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others.
Ligand Receptor
αEβ7 integrin
Bioactivity
Measured by its ability to support the adhesion of MCF-7 cells.
Cell Type
Embryonic Stem Cells, Epithelial cells, Erythrocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules
Antigen References
  1. Guillot C, et al. 2013. Science. 340: 1185.
  2. Yamada S, et al. 2005. Cell. 123: 889.
  3. Tunggal JA, et al. 2005. EMBI J. 24: 1146.
  4. Huber AH, et al. 2001. Cell. 105: 391.
  5. Nagafuchi A, et al. 1987. Nature. 329: 341.
  6. Cepek KL, et al. 1994. Nature. 372: 190.
  7. Butz S, et al. 1994. FEBS Letters. 355: 195.
  8. Fukunaga Y, et al. 2005. Cell Struct Funct. 30: 25.
  9. Gumbiner BM. 2005. Nat Rev Mol Cell Biol. 6: 622.
  10. Overduin M, et al. 1995. Science. 267:386.
  11. Boggon TJ, et al. 2002. Science. 296:1303.
  12. Berx G, et al. 1995. EMBO J. 14:6107.
  13. Perl AK, et al. 1998. Nature. 392:190.
  14. Ghoumid J, et al. 2017. Genet Med. 19: 1013.
  15. Hakuno M, et al. 2000. Br J Dermatol. 142: 702.
Gene ID
999 View all products for this Gene ID
UniProt
View information about E-Cadherin on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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