Recombinant Human DKK-1 (carrier-free)

Pricing & Availability
Other Names
Dickkopf-related protein 1, DKK1, Dickkopf WNT signaling pathway inhibitor 1
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Product Citations
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Human_DKK-1_CF_1_111518
Recombinant Human DKK-1 inhibits TCF reporter activity induced by 50 ng/mL of mouse Wnt3a (Cat. No. 772301) in a dose dependent manner. ED50 for this effect is 6 – 30 ng/mL.
  • Human_DKK-1_CF_1_111518
    Recombinant Human DKK-1 inhibits TCF reporter activity induced by 50 ng/mL of mouse Wnt3a (Cat. No. 772301) in a dose dependent manner. ED50 for this effect is 6 – 30 ng/mL.
  • Human_DKK-1_CF_2_111518
    Stability testing for Human DKK-1. Human DKK-1 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaws), and compared to a control kept at 4°C (Control). The samples were tested for their ability to inhibit TCF reporter activity induced by 50 ng/mL of mouse Wnt3a (Cat. No. 772301).
Cat # Size Price Quantity Avail. Save
778602 10 µg £124
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778604 25 µg £208
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778606 100 µg £638
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Description

DKK-1 (Dickkopf related protein 1) is a member of the Dickkopf family of proteins that include DKK-1, -2, -3,-4, and Soggy. DKK-1 was discovered by its ability to block Wnt signaling during early Xenopus embryogenesis. DKK-1 antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt proteins. DKK-1 forms a ternary complex with LRP5/6 and the transmembane protein Kremen1/2, promoting internalization of LRP5/6 and down regulation of Wnt signaling. DKK-1 contains two conserved cysteine-rich domains separated by a linker region. The N-terminal cysteine-rich domain is unique to DKKs while the C-terminal cysteine-rich domain has a pattern of 10 cysteines similar to colipase. DKK-1 binds with LRP6 with high affinity and this interaction is enhanced by Kremens. The interactions between DKK-1 and both LRP6 and Kremens are mediated via C-terminal cysteine-rich domain. DKKs are expressed throughout development and play an important role by locally inhibiting Wnt regulated processes such as limb development, somitogenesis, antero-posterior axial patterning, and eye formation. In the adult, DKKs are implicated in bone formation and bone disease, cancer and Alzheimer's disease. The balance between Wnt signaling and DKK-1 inhibition is important for bone homeostasis. Increased expression of DKK-1 are found in lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers. DKK-1 expression is decreased in malignant melanoma and colorectal cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human DKK-1, amino acid Thr32-His266 (Accession #O94907.1) was expressed in 293E cells.
Molecular Mass
The 235 amino acid recombinant protein has a predicted molecular mass of approximately 25.78 kD. The DTT-reduced and non-reduced protein migrates at approximately 36-45 kD by SDS-PAGE. The predicted N-terminal amino acid is Thr.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
Measured by its ability to inhibit mWnt3a (Cat. No. 772301) induced TCF reporter activity in HEK293T human embryonic kidney cells. ED50 = 6 – 30 ng/mL.
Application

Bioassay

Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution

Placenta, prostate, colon, spleen, and platelets

Function
Wnt antagonist implicated in bone formation and bone disease, cancer and Alzheimer's disease. Also promotes type 2 cell-mediated inflammation.
Interaction
Bone
Ligand Receptor
LRP5/6, Kremen-1, and Kremen-2.
Bioactivity
Inhibits mWnt3a activity in TCF reporter assay
Biology Area
Stem Cells, Cancer Biomarkers, Neuroscience, Neuroinflammation
Antigen References
  1. Fedi P, et al. 1999. J Biol Chem. 274:19465-72.
  2. Krupnik VE, et al. 1999. Gene. 238:301-13.
  3. Mao B, et al. 2001. Nature. 411:321-325.
  4. Mukhopadhyay M, et al. 2001. Dev Cell. 1:423-434.
  5. Tian E, et al. 2003. New Eng J Med. 349:2483-2494.
  6. Niehrs C. 2006. Oncogene. 25:7469-7481.
  7. Diarra D, et al. 2007. Nature Med. 12:156-163.
  8. Fujita K and Janz S. 2007. Molecular Cancer. 6:71.
  9. Ahn VE, et al. 2011. Dev Cell. 21:862-873.
Gene ID
22943 View all products for this Gene ID
UniProt
View information about DKK-1 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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