Recombinant Human CXADR/CAR-Fc Chimera (carrier-free)

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Other Names
Coxsackievirus and Adenovirus Receptor, CAR, CVB3 binding protein, Coxsackievirus B Receptor
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Human_CXADR-CAR_RECOM_Fc_CF_1_111518
When recombinant human CXADR/CAR-Fc Chimera is immobilized at 1 µg/mL, biotinylated recombinant mouse JAML binds with EC50 of 9 – 36 ng/mL in a functional ELISA.
  • Human_CXADR-CAR_RECOM_Fc_CF_1_111518
    When recombinant human CXADR/CAR-Fc Chimera is immobilized at 1 µg/mL, biotinylated recombinant mouse JAML binds with EC50 of 9 – 36 ng/mL in a functional ELISA.
  • Human_CXADR-CAR_RECOM_Fc_CF_2_111518
    Stability testing for recombinant human CXADR/CAR-Fc Chimera. Recombinant human CXADR/CAR-Fc Chimera was aliquoted in PBS, pH 7.2 at 0.2 mg/ml. One aliquot was freeze and thawed four times (4x freeze/thaws), and compared to a control kept at 4°C (control). The samples were tested in a binding assay with biotinylated recombinant mouse JAML.
Cat # Size Price Quantity Avail. Save
777402 10 µg £50
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777404 25 µg £94
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777406 100 µg £370
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777408 500 µg £874
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Description

CXADR (coxsackie and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (IGSF). Its 218 amino acid extracellular domain consists of two immunoglobulin-like domains, V-type (D1) and C-type (D2) that share 90% amino acid sequence identity with mouse and rat CXADR. CXADR functions as a receptor for group B coxsackieviruses and subgroup C adenoviruses. It also functions as a homophilic cell adhesion molecule within junctional complexes between epithelial cells lining body cavities. CXADR is essential for tight junction integrity and normal cardiac development in mice. It is also involved in transepithelial migration of leukocytes through interaction with JAML. The CXADR/JAML interaction activates gamma-delta T-cells through PI3-kinase and MAP kinase pathways. T-cell activation and subsequent proliferation leads to cytokine production stimulating epithelial tissue repair.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human CXADR, amino acid Leu20-Gly237 (Accession # P78310), with a C-terminal human IgG1 (Pro100-Lys330) was expressed in 293E cells.
Molecular Mass
The 457 amino acid recombinant protein has a predicted molecular mass of approximately 50.9 kD. The DTT-reduced and non-reduced protein migrates at approximately 55 kD and 110 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Leu.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
When recombinant human CXADR/CAR-Fc Chimera is immobilized at 1 µg/mL, biotinylated recombinant mouse JAML binds with EC50 of 9 – 36 ng/mL in a functional ELISA.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution

Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung.

Function
Component of the epithelial apical junction complex that functions as a homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with JAML.
Interaction
Epithelia cells, Leukocytes
Ligand Receptor
Coxsackievirus, Adenovirus, JAML
Bioactivity
Measured by its ability to bind biotinylated recombinant mouse JAML
Antigen References
  1. Bergelson JM, et al. 1997. Science. 275:1320-1323
  2. Tomko RP, et al. 1997. Proc Nat Acad Sci. 94:3352-3356
  3. Pauschinger M, et al. 1999. Circulation. 99:1348-1354
  4. Cohen CJ, et al. 2001. Proc Nat Acad Sci. 98:15191-15196
  5. Walters RW, et al. 2002. Cell. 110:789-799
  6. Zen K, et al. 2005. Mol Biol Cell. 16:2694-2703
  7. Verdino P, et al. 2010. Science. 329:1210-1214
  8. Witherden DA, et al. 2010. Science. 329:1205-1210
Gene ID
1525 View all products for this Gene ID
UniProt
View information about CXADR on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 11/16/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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