Recombinant Human Cripto (carrier-free)

Pricing & Availability
Other Names
Teratoma-derived growth factor 1 (TDGF-1), Epidermal Growth Factor-Like Cripto Protein (CR1), Cripto-1 Growth Factor, CR, CRGF, CR-1
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Human_Cripto_RECOM_CF_1_110718_updated
Recombinant Human Cripto binds to immobilized recombinant human ALK4 (at 1 µg/mL) in a dose dependent manner. The ED50 = 10 to 50 ng/mL.
  • Human_Cripto_RECOM_CF_1_110718_updated
    Recombinant Human Cripto binds to immobilized recombinant human ALK4 (at 1 µg/mL) in a dose dependent manner. The ED50 = 10 to 50 ng/mL.
  • Human_Cripto_RECOM_CF_2_110718_updated
    Stability testing for human Cripto. Human Cripto was aliquoted in DPBS at 0.2 mg/mL and one aliquot was kept at 4°C (control) and another was frozen and thawed four times (4X Freeze/Thaws). After this procedure, the samples were tested by binding assay. Immobilized human recombinant human ALK4 at 1 µg/mL, binds recombinant human Cripto in a dose dependent manner.
Cat # Size Price Quantity Avail. Save
777802 10 µg £77
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777804 25 µg £138
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Description

Cripto is a glycosylphosphatidylinositol (GPI)-anchored Type I cell membrane protein that belongs to the epidermal growth factor (EGF)-Cripto-1-FRL-1-Cryptic (CFC) family of proteins (EGF-CFC). In addition to Cripto, another three members of the EGF-CRC family have been described: Criptic, Xenopus FRL-1, and zebrafish one-eyed pinhead (0EP). Cripto contains a signal sequence, an EGF-like domain, a cysteine-rich region motif (CFC domain), and a hydrophobic COOH-terminus. Cripto plays an important role in maintaining embryonic stem cell pluripotency and tumorigenesis. Cripto acts as a co-receptor for Nodal, a TGFb family member that plays a crucial role in mesoderm and endoderm formation. Nodal and Cripto form complex with type I Activin receptors, Alk4/7 (ACVR1B/ACVR1C), and type II Activin receptors, ActRIIA/ActRIIB (ACVR2A/ACVR2B). Cripto is also known to bind Furin and Pace4 that process Nodal proteolytic maturation and supports Nodal signaling. A soluble form of Cripto is secreted by tumor cells and it lacks the GPI anchor region which is removed by GPI-phospholipase D. The soluble Cripto promotes endothelial cell migration and possibly tumor angiogenesis. More recent data showed that a GPI phospholipase A2 called post-glycosylphosphatidylinositol attachment to proteins 6 (PGAP6) in involved in the in the shedding of Cripto. PGAP6 produces Cripto bearing lyso-GPI which is released from the membrane, and subsequently Cripto is released from the lyso–GPI by GPI-phospholipase D.  The action of PGAP6 regulates Nodal signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human Cripto, amino acids Leu31 - Thr172 (Accession # AAH67844) was expressed in 293E cells. The carboxy-terminal contins TG-8His-GGQ tag.
Molecular Mass
The 155 amino acid recombinant protein has a predicted molecular mass of approximately 17.4 kD. The DTT-reduced and non reduced protein migrates at approximately at 25 - 30 kD by SDS-PAGE. The predicted N-terminal amino acid is Leu.
Purity
>95 %, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is in DPBS, pH 7.2
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
Recombinant human Cripto binds to immobilized recombinant human ALK4 ( at 1 µg/mL) in a dose dependent manner. The ED50 = 10 to 50 ng/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution

Mainly expressed during embryogenesis, embryonic stem cells, induced in pluripotent stem cells. Low expression in adult tissues, hematopoetic stem cells, mammary epithelial cells, adipose tissue, pancreas, and endometrium.

Function
Induces cell growth, migration, invasion, and epithelial to mesenchymal transition. Participates in tumorigenesis, cancer cell proliferation and survival.
Interaction
Interacts with Nodal, ALK4, Furin, PACE4, and GRP78.
Ligand Receptor
Co-receptor of Nodal, GDF1, and GDF3
Bioactivity
Measured by its ability to bind to human ALK4
Biology Area
Stem Cells, Cancer Biomarkers
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References
  1. Ciccodicola A, et al. 1989. EMBO J. 8:1987.
  2. Beck S, et al. 2002. Nature Cell Biology. 4:981.
  3. Gray PC, et al. 2003. PNAS. 100: 5193.
  4. Xing PX, et al. 2004. Cancer Res. 64:4018.
  5. Watanabe K, et al. 2007. J Biol Chem.  282:35772.
  6. Blanchet MH, et al. 2008. EMBO J. 27:2580.
  7. Gray PC and Vale W, 2012. FEBs Letter. 586:1836.
  8. Lee GH, et al. 2016. J Cell Biol. 215:705.
  9. Zoni E, et al. 2017. Oncogene. 36:4739.
Gene ID
6997 View all products for this Gene ID
UniProt
View information about Cripto on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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