Recombinant Human CD326/EPCAM (carrier-free)

Pricing & Availability
Other Names
Cluster of differentiation 326, CD326, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, TROP1, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, M1S2, M4S1, MIC18, TACSTD1
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Product Citations
publications
Human_CD326_RECOM_1_101818
Recombinant human CD326/EPCAM supports the adhesion of L929 cells in a dose dependent manner. ED50 for this effect is 0.4 - 1.6 µg/mL. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells.
  • Human_CD326_RECOM_1_101818
    Recombinant human CD326/EPCAM supports the adhesion of L929 cells in a dose dependent manner. ED50 for this effect is 0.4 - 1.6 µg/mL. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells.
  • Human_CD326_RECOM_2_101818
    Stability testing for recombinant human CD326/EPCAM. Recombinant human CD326/EPCAM was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x freeze/thaws), and compared to a control kept at 4°C (control). The samples were tested in an adhesion assay with L929 cells.
Cat # Size Price Quantity Avail. Save
777302 10 µg £50
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777304 25 µg £111
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777306 100 µg £292
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Description

EPCAM is a type I transmembrane protein, composed of thyroglobulin type I domain on the extracellular domain, a transmembrane region and a 26-amino acid intracellular region. EPCAM is expressed in epithelial tissues, but it cannot be detected in normal squamous epithelia except carcinoma. In mice, the homolog of human EPCAM has been found in thymic epithelial cells, thymocytes, T cells and dendritic cells. The expression level of EPCAM differs among tissues, but it is higher in proliferating cells than in differentiated cells. Several studies demonstrated that EPCAM is involved in cell adhesion, proliferation, migration and gene expression. EPCAM mediates homophilic cell-cell adhesions in Ca2+-independent manner. Its N-terminal domain is necessary for adhesion.  In addition, EPCAM serves as a diagnostic biomarker of cancer. High level of EPCAM is found in several carcinoma and correlates with tumor progression in cervical neoplasia and survival in breast cancer patients. EPCAM associates with the tight junction protein claudin 7, resulting in tumor growth, metastasis formation and apoptotic resistance. EPCAM undergoes intramembrane proteolysis, catalyzed by TNF-α converting enzyme (TACE) and presenilin-2 (PS-2/γ-secretase), which in turn produces the extracellular (EpEX) and intracellular (EpICD) domains of EPCAM. EpICD forms a nuclear complex with β-catenin, its co-activator FHL2 and Lef-1. It has been indicated that EPCAM upregulates oncogenic transcriptional factor c-myc and cell cycle-related proteins Cyclin A/E. Furthermore, EPCAM can induce expression of reprogramming factors, including OCT4, SOX2 and NANOG. EPCAM is a potential target in cancer therapy. Antibodies against EPCAM and vaccine-based clinical trials in different carcinoma have been conducted.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human EPCAM, amino acid Gln24-Lys265 (Accession # P16422), with 6His tag, was expressed in 293E cells.
Molecular Mass
The 248 amino acid recombinant protein has a predicted molecular mass of approximately 28.2 kD. The DTT-reduced and non-reduced protein migrates at approximately 36.5 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Gln.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 μm filtered protein solution is in PBS, pH 7.2
Concentration
10-25 µg sizes are bottled at 200 µg/mL. 100 µg size is lot-specific and bottled at the concentration indicated on the vial. (Please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Application References

(PubMed link indicates BioLegend citation)
  1. Balzar M, et al. 2001. Mol. Cell. Biol. 21:2570.
  2. Nelson AJ, et al. 1996. Eur. J. Immunol. 26:401.
  3. Schnell U, et al. 2013. Biochim. Biophys. Acta. 8:1989.
  4. Nübel T, et al. 2009. Mol. Cancer Res. 7:285.
  5. Münz M, et al. 2004. Oncogene 23:5748.
  6. Philip R, et al. 2015. Oncotarget. 6:2046.
  7. Maetzel D, et al. 2009. Nat. Cell. Biol. 11:162.
  8. Armstrong A, Eck SL. 2003. Cancer Biol. Ther. 2:320.
  9. Lin CW, et al. 2012. J. Biol. Chem. 287:39449.

Antigen Details

Structure
Monomer
Distribution

Undifferentiated embryonic stem cells, epithelial cells, adenocarcinoma; Plasma membrane

Function
EPCAM mediates Ca2+-independent homophilic intercellular adhesion and it is involved in tumor progression.
Interaction
Claudin 7, β-catenin, FHL2
Ligand Receptor
EPCAM
Bioactivity
Measured by its ability to support the adhesion of L929 cells
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Gene ID
4072 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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