Recombinant Human BTLA-Fc Chimera (carrier-free)

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Other Names
CD272, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein
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BTLA-Fc_Chimera_CF_RECOM_1_082118
Immobilized recombinant human BTLA at 1.0 µg/mL binds recombinant mouse HVEM in a dose dependent manner with EC50 of 7 - 21 ng/mL.
  • BTLA-Fc_Chimera_CF_RECOM_1_082118
    Immobilized recombinant human BTLA at 1.0 µg/mL binds recombinant mouse HVEM in a dose dependent manner with EC50 of 7 - 21 ng/mL.
  • BTLA-Fc_Chimera_CF_RECOM_2_082118
    Stability testing for Human BTLA. Human BTLA was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was freeze thawed four times (4x freeze/thaws), and compared to a control kept at 4°C (control). The samples were tested for their ability to bind mouse HVEM in a functional ELISA.
Cat # Size Price Quantity Avail. Save
775702 10 µg £38
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775704 25 µg £55
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775706 100 µg £195
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Description

BTLA (B and T lymphocyte attenuator, CD272) is a type I transmembrane glycoprotein with four extracellular cysteine-rich domains. It is an inhibitory molecule structurally and functionally similar to cytotoxic T lymphocyte antigen-4 (CTLA-4) and programmed death-1 (PD-1).  These molecules belong to the immunoglobulin superfamily that binds B7 family members. Different from CTLA-4 and PD-1 binding with the B7 family members, BTLA interacts with herpes virus entry mediator (HVEM) which belongs to TNFR family. BTLA/HVEM interaction was the first demonstration of crosstalk between these two family members.  BTLA/HVEM interaction negatively modulates T cell activation. HVEM induces BTLA tyrosine phosphorylation and inhibits T-cell proliferation.  There is accumulating evidence suggesting that BTLA/HVEM signaling also plays an important role in autoimmunity and infection at mucosal surfaces. The BTLA/HVEM interaction does not prevent the simultaneous binding of other HVEM ligands such as LIGHT or Lymphotoxin-alpha. BTLA is expressed on T cells, B cells, macrophages, dendritic cells, and natural killer cells. Constitutive expression level of BTLA on resting T cells are very low, but increase after the activation of T cells. BTLA is highly expressed on resting B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human BTLA, amino acids Lys31-Trp153 (Accession # Q7Z6A9) with a C-terminal human IgG1 (Pro100-Lys330) Fc tag expressed in CHO cells.
Molecular Mass
The 362 amino acid recombinant protein has a predicted molecular mass of approximately 41 kD. The DTT-reduced protein migrates at approximately 55 kDa and and non-reduced protein migrates at approximately 110 kDa by SDS-PAGE. The predicted N-terminal amino acid is Lys.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
When human BTLA is immobilized at 1.0 µg/mL (100 µL/well), recombinant mouse HVEM binds with EC50 of 7 - 21 ng/mL in a functional ELISA.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution

T cells, B cells, macrophages, dendritic cells, and natural killer cells.

Function
Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response
Interaction
Lymphocytes
Ligand/Receptor
HVEM
Bioactivity
Measured by its ability to bind mouse HVEM
Cell Targets
Lymphocytes
Cell Type
B cells, Dendritic cells, Tregs
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
Molecular Family
Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Watanabe N, et al. 2003. Nat. Immunol. 4: 670-9
  2. Gavrieli M, et al. 2003. Biochem. Biophys. Res. Commun. 312: 1236-43
  3. Gonzalez LC, et al. 2005. Proc. Natl. Acad. Sci. U S A 102: 1116-21
  4. Sedy JR, et al. 2005. Nat. Immunol. 6: 90-98
  5. Murphy KM, et al.  Nat. Rev. Immunol. 6: 671-81
Gene ID
151888 View all products for this Gene ID
UniProt
View information about BTLA on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 08/22/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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