Recombinant Human Artemin (carrier-free)

Pricing & Availability
Regulatory Status
Other Names
ARTN, ART, Enovin, Neublastin, EVN, NBN
Ave. Rating
Submit a Review
Product Citations
When human Artemin is immobilized at 1.0 µg/mL, human GFR alpha3-Fc Chimera binds with EC50 of 20 - 80 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.
  • When human Artemin is immobilized at 1.0 µg/mL, human GFR alpha3-Fc Chimera binds with EC50 of 20 - 80 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.
  • Stability testing for Human Artemin. Human Artemin was aliquoted in 4 mM HCl at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested for their ability to bind human GFR alpha3-Fc Chimera.
Cat # Size Price Quantity Check Availability Save
780202 10 µg £41
Check Availability

Need larger quantities of this item?
Request Bulk Quote
780204 25 µg £94
Check Availability

Need larger quantities of this item?
Request Bulk Quote
780206 100 µg £262
Check Availability

Need larger quantities of this item?
Request Bulk Quote

Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.


Artemin belongs to the GDNF family of ligands consisting of 4 members: GDNF, Neurturin, Artemin, and Persephin. GDNF family members orchestrate multiple aspects of the development and maintenance of the central and peripheral nervous systems. These GDNF family ligands signal through a multicomponent receptor system composed of a common signaling component RET (receptor tyrosine kinase) and one of the high affinity binding coreceptors GFRα (α1-α4). Artemin is a GDNF family member that functions as a ligand for the GFRα3 and RET complex. Artemin is a disulfide-linked homodimer that contains three intra-chain disulfide linkages and a cysteine knot motif. Artemin supports the survival of sensory and sympathetic peripheral neurons and also supports survival of dopaminergic midbrain neurons. Artemin expression in adult tissues is ubiquitous with high levels in peripheral tissues including the prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis. In adult rat, systemic artemin treatment promoted the regeneration of sensory axons to the brainstem after brachial dorsal root crush. Recent findings suggest Artemin regulates multiple aspects of carcinoma progression and metastasis. Artemin is a hypoxia-responsive factor and is essential for hypoxia-induced cancer stem cell expansion in hepatocellular carcinoma and Artemin stimulates de novo tumor angiogenesis in mammary carcinoma mediated in part by VEGF-A.  

Product Details
Technical Data Sheet (pdf)

Product Details

Human Artemin, amino acid Ala108-Gly220 (Accession # Q5T4W7), was expressed in E.coli.
Molecular Mass
The 113 amino acid recombinant protein has a predicted molecular mass of approximately 12 kD. The DTT-reduced proteins migrate at approximately 15 kD, and non-reduced proteins migrate at approximately 30 kD by SDS-PAGE. The predicted N-terminal amino acid is Ala.
>95%, as determined by Coomassie stained SDS-PAGE.
0.22 µm filtered protein solution is in 4 mM HCl
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
When human Artemin is immobilized at 1.0 µg/mL, human GFR alpha3-Fc Chimera binds with EC50 of 20 - 80 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.


Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at

Antigen Details

Disulfide-linked homodimer

Ubiquitous with high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis.

Supports the survival of sensory and sympathetic neurons, and also dopaminergic midbrain neurons.
Sensory neurons, sympathetic neurons, midbrain dopaminergic neurons.
GFRα3, GFRα1
Measured by its ability to bind GFRα3
Cell Type
Neural Stem Cells, Embryonic Stem Cells
Biology Area
Cell Biology, Stem Cells
Molecular Family
Adhesion Molecules, Growth Factors
Antigen References
  1. Baloh RH, et al. 1998. Neuron. 21:1291-1302
  2. Masure S, et al. 1999. Eur J Biochem. 266:892-902
  3. Gardell LR, et al. 2003. Nature Med. 9:1383-1389
  4. Honma Y, et al. 2002. Neuron. 35:267-282
  5. Arindam B, et al. 2012. PLoS One. 7(11):e50098
  6. Wong LE, et al. 2015. Proc Natl Acad Sci USA. 112:6170-5
  7. Zhang M, et al. 2016. Oncotarget. 7:3267-82
Gene ID
9048 View all products for this Gene ID
View information about Artemin on

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 01/29/2019

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account