- Other Names
- ARTN, ART, Enovin, Neublastin, EVN, NBN
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- Product Citations
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Artemin belongs to the GDNF family of ligands consisting of 4 members: GDNF, Neurturin, Artemin, and Persephin. GDNF family members orchestrate multiple aspects of the development and maintenance of the central and peripheral nervous systems. These GDNF family ligands signal through a multicomponent receptor system composed of a common signaling component RET (receptor tyrosine kinase) and one of the high affinity binding coreceptors GFRα (α1-α4). Artemin is a GDNF family member that functions as a ligand for the GFRα3 and RET complex. Artemin is a disulfide-linked homodimer that contains three intra-chain disulfide linkages and a cysteine knot motif. Artemin supports the survival of sensory and sympathetic peripheral neurons and also supports survival of dopaminergic midbrain neurons. Artemin expression in adult tissues is ubiquitous with high levels in peripheral tissues including the prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis. In adult rat, systemic artemin treatment promoted the regeneration of sensory axons to the brainstem after brachial dorsal root crush. Recent findings suggest Artemin regulates multiple aspects of carcinoma progression and metastasis. Artemin is a hypoxia-responsive factor and is essential for hypoxia-induced cancer stem cell expansion in hepatocellular carcinoma and Artemin stimulates de novo tumor angiogenesis in mammary carcinoma mediated in part by VEGF-A.Product Details
- Human Artemin, amino acid Ala108-Gly220 (Accession # Q5T4W7), was expressed in E.coli.
- Molecular Mass
- The 113 amino acid recombinant protein has a predicted molecular mass of approximately 12 kD. The DTT-reduced proteins migrate at approximately 15 kD, and non-reduced proteins migrate at approximately 30 kD by SDS-PAGE. The predicted N-terminal amino acid is Ala.
- >95%, as determined by Coomassie stained SDS-PAGE.
- 0.22 µm filtered protein solution is in 4 mM HCl
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
- When human Artemin is immobilized at 1.0 µg/mL, human GFR alpha3-Fc Chimera binds with EC50 of 20 - 80 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at firstname.lastname@example.org.
- Disulfide-linked homodimer
Ubiquitous with high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis.
- Supports the survival of sensory and sympathetic neurons, and also dopaminergic midbrain neurons.
- Sensory neurons, sympathetic neurons, midbrain dopaminergic neurons.
- Ligand Receptor
- GFRα3, GFRα1
- Measured by its ability to bind GFRα3
- Biology Area
- Cell Biology
- Molecular Family
- Adhesion Molecules, Growth Factors
- Antigen References
- Baloh RH, et al. 1998. Neuron. 21:1291-1302
- Masure S, et al. 1999. Eur J Biochem. 266:892-902
- Gardell LR, et al. 2003. Nature Med. 9:1383-1389
- Honma Y, et al. 2002. Neuron. 35:267-282
- Arindam B, et al. 2012. PLoS One. 7(11):e50098
- Wong LE, et al. 2015. Proc Natl Acad Sci USA. 112:6170-5
- Zhang M, et al. 2016. Oncotarget. 7:3267-82
- Gene ID
- 9048 View all products for this Gene ID
- View information about Artemin on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.