Recombinant Human Apolipoprotein H/ApoH (carrier-free)

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Other Names
Apolipoprotein H, APOH, Apo-H, Beta-2-glycoprotein 1, B2GPI, Beta(2)GPI, BG, APC inhibitor, Activated protein C-binding protein, Anticardiolipin cofactor
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Human_ApoH_CF_RECOM_1_031519
When human Apolipoprotein H/ApoH is coated at 2 µg/mL (100 µL/well), human LDLR (Cat. No. 555304) binds with an EC50 of 0.075 – 0.3 µg/mL in a functional ELISA.
  • Human_ApoH_CF_RECOM_1_031519
    When human Apolipoprotein H/ApoH is coated at 2 µg/mL (100 µL/well), human LDLR (Cat. No. 555304) binds with an EC50 of 0.075 – 0.3 µg/mL in a functional ELISA.
  • Human_ApoH_CF_RECOM_2_031519
    Stability testing for Human Apolipoprotein H/ApoH. Recombinant human Apolipoprotein H/ApoH was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (control). The samples were tested in a binding assay with human LDLR.
Cat # Size Price Quantity Avail. Save
781602 10 µg £51
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781604 25 µg £113
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781606 100 µg £239
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Description

Apolipoprotein H (ApoH) is a single-chain plasma protein of about 50 kD. It is a member of the complement control superfamily of proteins. ApoH is also known as Beta-2 glycoprotein and Activated protein C-binding protein. ApoH has been implicated in a variety of physiologic pathways, including blood coagulation, hemostasis, and the production of antiphospholipid antibodies characteristic of antiphospholipid syndrome. ApoH binds negatively charged phospholipid macromolecules and circulates as a component of lipoprotein particles and as a lipid-free serum protein. ApoH may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. ApoH also inhibits thrombosis by blocking the activation of Coagulation Factor XI but also shows pro-coagulant activity by inhibiting the activation of Protein C. The plasma concentrations of ApoH are strongly associated with metabolic syndrome alterations and vascular disease in type 2 diabetics and could be considered as a clinical marker of cardiovascular risk.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human ApoH, amino acid Gly20-Cys345 (Accession # P02749) was expressed in 293E cells.
Molecular Mass
The 326 amino acid recombinant protein has a predicted molecular mass of approximately 36.3 kD. The DTT-reduced protein migrates at approximately 50 kD and and non-reduced protein migrates 45 kD by SDS-PAGE. The predicted N-terminal amino acid is Gly.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
When human Apolipoprotein H/ApoH is coated at 2 µg/mL (100 µL/well), human LDLR (Cat. No. 555304) binds with an EC50 of 0.075 – 0.3 µg/mL in a functional ELISA.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution

Expressed by the liver and secreted in plasma.

Function
Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate.
Ligand/Receptor
Heparin, phospholipids, dextran sulfate
Bioactivity
Measured by its ability to bind human LDLR
Biology Area
Cell Biology
Molecular Family
Apolipoproteins
Antigen References
  1. Hoeg JM, et al. 1985. Atherosclerosis. 55: 25-34
  2. Bancsi LF, et al. 1992. Thromb Haemost. 67: 649-653
  3. Chonn A, et al. 1995. J Biol Chem. 270: 25845-25849
  4. Sheng Y, et al. 2001. J Biol Chem. 276: 13817-13821
  5. Shi T, et al. 2004. Proc Natl Acad Sci. 101: 3939-3944
  6. Castro A, et al. 2010. Atherosclerosis. 209: 201-205
  7. Giannakopoulos B, et al. 2011. Curr Rheum Rep. 13: 90-95
Gene ID
350 View all products for this Gene ID
UniProt
View information about Apolipoprotein H on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 2    Revision Date: 05/03/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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