Purified F(ab')2 Goat anti-human IgG, IgM (H+L chain) Antibody

Pricing & Availability
Clone
Poly23973 (See other available formats)
Isotype
Goat Polyclonal Ig
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Product Citations
publications
Poly23973_PURE_IgG_IgM_Antibody_1_090319
Human peripheral blood lymphocytes were stimulated with 10 µg/mL of Purified F(ab')2 Goat anti-human IgG, IgM (H+L chain) antibody, clone Poly23973 (left) or unstimulated (right) for 24 hours. Cells were surface stained with CD19 APC and CD69 PE.
  • Poly23973_PURE_IgG_IgM_Antibody_1_090319
    Human peripheral blood lymphocytes were stimulated with 10 µg/mL of Purified F(ab')2 Goat anti-human IgG, IgM (H+L chain) antibody, clone Poly23973 (left) or unstimulated (right) for 24 hours. Cells were surface stained with CD19 APC and CD69 PE.
  • Poly23973_PURE_IgG_IgM_Antibody_2_083019
    Human peripheral blood lymphocytes were stimulated with 10 µg/mL of Purified F(ab')2 Goat anti-human IgG, IgM (H+L chain) antibody, clone Poly23973 (left) or unstimulated (right) for 2 minutes, fixed with Fixation buffer, permeabilized with Intracellular Staining Permeabilization Wash Buffer, then stained with CD20 APC and ZAP70 Phospho (Tyr319)/Syk (Tyr352) PE.
Cat # Size Price Quantity Avail. Save
397302 500 µg £165
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Description

Poly23973 reacts with both human IgG and IgM. It can be used in functional assays, such as B cell activation. It has minimum cross-reactivity with bovine proteins. The optimal working concentration should be determined empirically based on the specific assay of interest.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Preparation
The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. The solution contains no preservative; handle under aseptic conditions.
Application

Activ - Quality tested

Recommended Usage

This product was QC tested by stimulating human peripheral blood B cell. The recommended working concentration is 10 - 20 µg/mL.

RRID
AB_2814370 (BioLegend Cat. No. 397302)

Antigen Details

Cell Type
B cells
Biology Area
Immunology, Signal Transduction
Molecular Family
Adaptor Proteins
Gene ID
3507 View all products for this Gene ID 3492 View all products for this Gene ID

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/30/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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