Purified anti-ZAP-70 Antibody

Pricing & Availability
Clone
A16043B (See other available formats)
Other Names
Zeta chain of TCR associated protein kinase 70
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
A16043B_PURE_ZAP-70_Antibody_052819.png
Human peripheral blood lymphocytes treated with True-Nuclear™ Transcription Factor Buffer Set, then stained with ZAP-70 (clone A16043B) Purified (filled histogram) or Purified Mouse IgG1, κ Isotype Ctrl (open histogram) followed by PE Goat anti-mouse IgG (minimal x-reactivity).
  • A16043B_PURE_ZAP-70_Antibody_052819.png
    Human peripheral blood lymphocytes treated with True-Nuclear™ Transcription Factor Buffer Set, then stained with ZAP-70 (clone A16043B) Purified (filled histogram) or Purified Mouse IgG1, κ Isotype Ctrl (open histogram) followed by PE Goat anti-mouse IgG (minimal x-reactivity).
Cat # Size Price Quantity Avail. Save
693502 100 µg £118
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

ZAP-70 is a 70kD protein expressed near the surface membrane of T-cells and NK cells. It is part of the intracellular signaling mechanism of the T-cell receptor, and plays integral role in T-cell signaling where it interacts with CD3-ζ to phosphorylate transmembrane protein LAT, which facilitates the binding of many signaling proteins. ZAP-70 plays a key role in the activation of T-cells, and their subsequent proliferation, differentiation, and production of cytokines in response to varied stimuli. It is used clinically as a prognostic marker helping differentiate the various types of chronic lymphocytic leukemia (CLL).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human ZAP 70 peptide phosphorylated at Y493. Complete Freund’s adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

CD3, CD19, and CD56 co-staining indicates that this clone does not bind B-cells and will not bind Syk. Clone A16043B cross-reacts with mouse species.

This clone, A16043B, recognizes both non-phosphorylated ZAP-70 and phosphorylated forms of ZAP-70.

Antigen Details

Structure
Protein tyrosine kinase, contains two SH2 domains, 70 kD.
Distribution

T cells, NK cells, and B-cell chronic lymphocytic leukemia (B-CLL).

Function
Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP70 causes selective T cell defect.
Ligand/Receptor
Binds to CD3 zeta chain, also associates with a variety of proteins including SLA, Fyn, RasGap, Lck, Vav1, and Shc.
Cell Type
Leukemia, NK cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
TCRs
Antigen References

1. Qian D, et al. 1997. J. Exp. Med. 185:1253. (ICFC)
2. Crespo M, et al. 2003. N. Engl. J. Med. 348:1764. (WB, IP)
3. Jamroziak K, et al. 2009. Cancer Epidemiol. Biomarkers Prev. 18:945.

Gene ID
7535 View all products for this Gene ID 22637 View all products for this Gene ID
UniProt
View information about ZAP-70 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/28/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account