Purified anti-Wnt3A Antibody

Pricing & Availability
Clone
BL28556 (See other available formats)
Other Names
Protein Wnt-3a
Isotype
Mouse IgG2b, κ
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Product Citations
publications
BL28556_Pure_Wnt3A_Antibody_011519_updated
Western blot of purified anti-Wnt3A antibody (clone BL28556). Lane 1: Molecular weight marker; Lane 2: 20 µg of HEK293 cell lysate; Lane 3: 20 µg of MCF-7 cell lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28556_Pure_Wnt3A_Antibody_011519_updated
    Western blot of purified anti-Wnt3A antibody (clone BL28556). Lane 1: Molecular weight marker; Lane 2: 20 µg of HEK293 cell lysate; Lane 3: 20 µg of MCF-7 cell lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
855601 25 µg £60
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Description

Wnt3a, one of Wnt family members, plays a key role in carcinogenesis. Studies have shown that Wnt3a can both promote and suppress cancer progression depending on the cancer type. Mechanistically, Wnt3a activates downstream signaling pathways in a canonical manner by binding to its receptor Frizzled (Fz) and co-receptor lipoprotein receptor-related proteins 5/6 (LRP5/6), which ultimately promote nuclear translocation of the transcription factor b-catenin. Wnt3A is also involved in non-oncogenic pathways. It modulates brain-derived insulin by increasing NeuroD1 expression levels. Following traumatic brain injury, Wnt3A secreted by mesenchymal stem cell was shown to be neuroprotective and promote neurocognitive recovery. 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
0.01 M PBS, pH 7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Wnt3A has 2 isoforms. Isoform 1 is a 352 amino acid protein with a molecular mass of ~40 kD. Isoform 2 is a 385 amino acid protein with a molecular mass of ~43 kD.
Distribution

Tissue Distribution: Lung, placenta, carcinoma tissues (breast cancer, colon cancer, gastric cancer, leukemia, and liver cancer).
Cellular distribution: Plasma membrane, cytosol, and extracellular.

Function
Wnt3a plays crucial role in carcinogenesis of various tissue types.
Interaction
Wnt3a binds to its receptor Frizzled (Fz) and co-receptor lipoprotein receptor-related proteins 5/6 (LRP5/6), and activates the canonical Wnt signaling pathway.
Ligand Receptor
Receptor: Frizzled (Fz); Coreceptor: Lipoprotein receptor-related proteins LRP5/6
Biology Area
Neuroscience, Synaptic Biology
Antigen References
  1. Lu, C. et al. 2017. Int. J. Oncol. 51(4):1135-1145.
  2. He, S. et al. 2015. Chin. J. Cancer. 34(12):554-62.
  3. Chen D, et al. 2014. Int J Clin Exp Pathol. 7(4), 1359–1368.
Gene ID
89780 View all products for this Gene ID
UniProt
View information about Wnt3A on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 01/10/2019

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